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We narrowed to 925 results for: Gene

Showing: 421 - 440 of 925 results

  1. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...address this issue by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The...the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the.... However, it was (and still is) infeasible to generate highly specific antibodies for every potential...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...epitope within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by...a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus... Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020....scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead...synthetase complex, the team investigated whether the generated antibodies could bind these aaRSs. They found ... Gräslund lab plans to continue their work by generating antibodies for additional aminoacyl tRNA synthetases...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...guides and an NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself On the other hand...were having difficulty achieving indel formation (gene disruption) with this new technology. To dig more...asked if they could detect indels (an indication of gene disruption ability) with NgAgo, one respondent out... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions...the water. On the bright side, successful indel generation and epitope knockin was achieved by a few respondents...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...adeno-associated viral vectors) are commonly used for in vivo gene delivery due to their low immunogenicity and range... the potential “sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...in AT-rich genomes. Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...certain tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...

  5. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors To focus on a particular gene or protein, check out our gene-specific biosensors. These constructs...Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration..., and voltage across membranes. These sensors generally follow the same pattern described above - a sensing...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...used to improve CRISPR-based activation of target genes, but we’ll save that application for another day...protein-tagging system for signal amplification in gene expression and fluorescence imaging.” Cell 159(3...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Truncated gRNAs for Regulating Gene Expression and CRISPR 101: Epigenetics and Editing the Epigenome (plus...Joining and PITCHing MMEJ as an Alternative Route for Gene Editing Day 9: Precision editing: Single Base Editing...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to design programmable... blue flame plasmids covering three different epigenetic effectors. The diversity of tools is especially...plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide...

  8. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...and caveats of Cre usage: Vagaries of conditional gene targeting. Schmidt-Supprian M, Rajewsky K. Nat Immunol...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167...specific recombination site for each enzyme, were generated to test recombination efficiency. When expressed....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...

  9. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... (Shmakov et al., 2015). They were using Cas1, a gene commonly associated with CRISPR arrays and involved...analysis, they identified 53 potential candidate genes that fell into 3 categories based on the architecture...expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although the...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check Out Our CRISPR Featured...27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering... PMC5127784. Find plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic ...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...inducible gene-expression sequences, and more. By combining these components, one can generate constructs...localization, as well as cellular processes like gene expression and cell migration (Sladitschek and Neveu... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation...to combine the functions of different genetic elements to generate non-natural tools such as novel biological...Resources on Addgene.org Browse our Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid...nutshell, is the systematic combination of different genetic sequences into plasmid DNA. In high throughput ...techniques, although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian...

  12. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...nucleus AAVs CREATEd for gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster...with the development of methodologies to deliver genes to the CNS have recently made it much easier to ..., and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied in ...essential for neuroscience research. The use of genetic tools for visualizing and perturbing circuits together...Adeno-associated virus (AAV) has been widely used to deliver genetic tools to the brain because of its low cytotoxicity...connections to that population. Limitations of common genetically encoded neuronal tracers Neuronal tracers such...influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived epitope (V5), the...

  13. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...-in mutations and gene editing Targeted genome editing events fall into two general classes: knock-in ...HDR. If your Cas9 cuts in the coding region of a gene, make sure that the PAM edit you introduce is a ...Guo, Y., Tian, Qigi, Lan, Y., et. al. An efficient gene knock-in strategy using 5’-modified dsDNA donors...translation by generating a frameshift mutation. The majority of cellular repair events will generate these types...family member which generates sticky ends upon cutting. Cas12a may promote HDR by generating ssDNA overhangs...Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you...cells, but a less frequent DNA repair mechanism in general. On average, it accounts for less than 10% of DNA...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using...26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals...27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary...

  15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic... (recognized by Flp), to either flip or excise a gene that is located between them. Early recombinase-...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277...Peripheral Nervous System and Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org/...end of the transgene. Since loxp and lox2272 can not recombine, this ensures that the transgene is no longer...flank a transgene (homologous pairs) or used different scrambled sequences to flank the transgenes (non-... express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination for every lot of...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...function better. In that case, gene shuffling might be a good option. In gene shuffling, you chop up your... this goal, including error-prone PCR, degenerate primers, gene shuffling, and structure-guided directed...mutate specific regions of your antibody. Gene shuffling Perhaps you like bits and pieces of an antibody but...sequences. Degenerate primers Using degenerate primers is similar to error-prone PCR. Degenerate primers ...resources on Addgene.org IPI Antibody Collection Antibody Guide Addgene Antibody Collection Addgene Antibody...the same general cyclical process (Figure 2). The differences typically come in when generating the library... 2: General process of antibody directed evolution. Created with BioRender.com.   Generating mutant...

  17. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...experiment. PITChing MMEJ as an Alternative Route for Gene Editing Michohomology-mediated end joining (MMEJ...template to increase efficiency of insertion and gene replacement. Care to share your favorite CRISPR ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing studies. These...desired edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list... to keep current with the latest and greatest! Addgene resources 3 Tips to Improve HDR Efficiency for ...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many ...2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520... exhaustive refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16...s0014-5793(02)02775-8 Additional resources on the Addgene blog Browse all of our fluorescent protein blog...Find other fluorescent biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...with genes central to the replication process removed. This means the vector can deliver a genetic payload...vectors to reproduce when experimentally necessary, genes for the required proteins are packaged in separate... More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral Protocols...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast...labs. Viral vectors are viruses that have been genetically modified to either limit or completely eliminate...10.1007/978-3-319-07758-1_2 More resources on the Addgene blog Viral Vectors 101: Viral Applications Viral...

  20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...sequence of a thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. ...nick-closing activity of bacteriophage T4 DNA ligase." Gene 76.2 (1989): 245-254. PubMed PMID: 2753355. 12. ...blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis...restriction cloning), adaptor ligation for next generation sequencing (NGS) library construction, and circularization...fragments end-to-end without the exo/polymerase generation of annealed complementary regions. Why use a... does fidelity mean for a ligase? DNA ligases generally prefer fully Watson-Crick base-paired dsDNA substrates...as a correct base pair. DNA ligases have been generally found to have a higher discrimination at the upstream...
Showing: 421 - 440 of 925 results