...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...

...women. Bicuspid aortic valve (an aortic valve that has two leaflets, instead of three) and acute aortic ...intracellular signaling,” says Hébert. Previous research has shown that GNB1 mutations lead to increased activity...

...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

...can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety... would not be able to do. We hope that this blog has answered all of your questions about polyhistidine...

...Cecchetelli The auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to...light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA...

... CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

...Further, Sanger sequencing has a lower detection limit of 50-20% (although this has been improved in some ...Joung 2016 with permission. Even though progress has been made with off-target prediction algorithms, ...

...phenotypes to study. In addition, the Sánchez lab has made it easier to perform advanced developmental ...into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and mix the purified...

...relatively confident that the gene from the test species has a similar function to the gene in the reference species...left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell biology...

...particles with polybrene, a cationic polymer. Polybrene has long been used to increase retroviral transduction...of mouse zygotes and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice...

...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene...

...AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where...research. To improve cell-type specificity of AAV, work has focused on modifying the outside protein shell, or...

...to host proteins. After the antibody of interest has been cloned into an expression plasmid, the plasmid...through open plasmid sharing, the CRISPR community has created hundreds of useful variants of Cas9. By providing...

...available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally...important — but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available...

...including the propagation of plasmids in bacteria — has many points at which small errors can be introduced...Catching and addressing QC issues Addgene’s QC team has developed a process for identifying, categorizing...

...categorization is complex Plasmid classification has shifted over the years, and new classifications are...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many...

...anyone who has ever worked to sequence DNA on NGS platforms will tell you, while the process has become much...

...your data to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your... doors Getting involved in science communication has been the most rewarding step I have taken in my career...

...syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically...

...training. Addgene executive director Joanne Kamens has written a 5-part blog on the topic:  http://blog....people mostly online and on monthly phone calls. This has its challenges as meeting in person is often much...