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We narrowed to 878 results for: IND

Showing: 421 - 440 of 878 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...copies of the transgene that will recombine independently, cells may acquire one of a variety of colors...extremely versatile technology as recombination can be induced in developing fish via Cre protein microinjection...microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as ...powerful tool for understanding cell proliferation. Inducible XFP expression in the Ubow mouse Another murine...CFP, and YFP. After crossing onto a tamoxifen-inducible ubiquitin background, each cell in the mouse that...Köhler, Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...single-component systems take advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous publications...light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light CRY2-CIB1 heterodimerizes...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic...substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30....CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection? Check ...

  3. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser impacts the magnitude...While flow cytometry can be used for virtually all kinds of experiments and research fields, for any experiment...After that, the next gates fully depend on your individual experiment. Practical example: lymphocytes in...cellular debris and other larger cells. Note, we don’t mind the warning message at the top, as our cells of ...strategy, I can, of course, also draw multiple individual gates around the populations of interest (Figure...of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square gates were...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome...rendering the resulting construct resistant to Cas9 induced cleavage. Summary of Site Directed Mutagenesis ...is removed by DpnI restriction digestion leaving behind only the mutagenized plasmid. Although it is commonly...reaction. Ideally, your primers should be free of palindromic and repetitive sequences, but if present, a minor...similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...on Addgene.org Find Synthetic Biology Plasmids Find Bacterial Expression Systems Find Plasmids for Protein...optimized for parameters such as induction temperature and length of induction, which will vary between different...gels containing Phos-Tag, a small molecule that binds specifically to phosphate moieties and promotes ...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...Advances in Cre-lox recombination have included inducible Cre, such as the tamoxifen-responsive CreERT, ...single-cell labeling is necessary to distinguish individual cells. The Brainbow-1.0 construct contains three...mice.) The combinatorial effect of multiple, independent recombination events leads to a rainbow of colors... with the vast array of colors needed to mark individual neurons, the system also suffered from a number...they screened a variety of fluorescent proteins to find those with ideal characteristics (low aggregation...Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, ...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...science funding, limitations to facilities and indirect costs, and increased tariffs present an unprecedented... of the cost of creating and verifying them independently — particularly valuable when budgets are constrained...preparations, and antibody production that labs may find increasingly difficult to maintain or access. These...applications, addressing the new funding structures and indirect cost caps more efficiently. Offering free educational... seeking and expanding our collaborations with industry and public and private partners to create additional...

  8. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...similarly to their untagged counterparts. These findings motivated Georgieva et al. to develop new tags...from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and IT10 expressed well...on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence and immunoprecipitation...for both applications. To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions...detrimental effects on cell size and growth rate, indicating that they compromised protein-protein interactions...

  9. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...protection against infectious agents. Several enzymes behind this immunity have already been discovered and ...coli bacteria strain. They found that Cas14 could bind and cleave a targeted sequence of ssDNA. Unlike ...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide...

  10. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...Advisor/PI who will also be a good mentor? Where can I find a Mentor? What is this I hear about “group” or “...“A good mentor has your goals/needs/situation in mind, rather than (or as well as) their own.” “Someone...mentors/advisors like Yoda? If so, you might want to find additional sources of wisdom. While somewhat lighthearted...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...using AAVs Resources on Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors... different parts of the brain is mediated via individual neurons. Neurons are composed of a cell body,...axons directly to the prefrontal cortex, but also indirectly via synapses to neurons in other brain regions...and aid in visualization of the morphology of individual neurons, including dendritic and axonal extensions...these neurons fluoresce in different colors. An individual neuron can then be traced throughout the brain...Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K.D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...have rapidly been developed. We asked the individuals behind some of these new platforms what their motivations...collaborations within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists...ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty, an Associate Professor...extremely important part of the future of research. To find other COVID-19 databases and resources, check out...

  13. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...spinal muscular atrophy, the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi plans on...concrete understanding of the molecular biology behind SMN1 and its interaction partners, Dr. Di Schiavi...suffering from this little-known disease. You can find more information about the disease on the Meothelioma...suffering from rare diseases. These underrepresented individuals deserve a voice. Check back with Addgene often...Countries with Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

  14. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...: 4045853.  Find other plant resources at Addgene Check out Addgene’s plant vectors! Find the plasmids...Agrobacterium tumifaciens. Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic...during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti ...References Sahoo, Dipak Kumar, Nrisingha Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene...

  15. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...different constructs and cell types. Second, Cre may induce recombination at pseudo- or cryptic loxP sites ...proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with...offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited normally...10.1073/pnas.1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, ...-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Loonstra...

  16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...maybe a tumor. They wanted to find something better. Transparency window That’s exactly what they reported... Verkhusha explained it to me, the transparency window of mammalian tissues is set by the properties of... all of them fluoresce outside the transparency window of mammalian tissues,” Verkhusha said. “We developed...near-infrared fluorescent proteins within this transparency window, so now, we could see deeper.” Adding to the toolbox...

  17. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...group needed for DNA replication, Rep78/68 binds the Rep binding element (RBE) in the ITRs, uses its endonuclease...N-terminal domain of Rep78 and Rep68 contains a DNA-binding and endonuclease domains, all four Rep proteins...onto an assembled capsid. Then Rep52/40, which also bind the capsid, “pumps” the genome into the capsid through...plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment Browse our AAV protocols...

  18. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Vectr has layers, which let you lock and hide individual vectors (shapes defined by 2D points connected... open source and available for desktop use for Windows, Mac OS X, and Linux. Inkscape is versatile and...biology, medical specialities, and general items. Individual images as well as collections of images are easy...appropriate credit, provide a link to the license, and indicate if changes were made to the images. This requirement...Toolbox blog post on social media for scientists Find science career blog posts Learn about other lab ...

  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...most common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which...system (bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature...while epitope tags are used for both direct and indirect visualization. Some of these tags can also be ...0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein as a Solubility Enhancer. In P. E. Vaillancourt...

  20. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...scientist’s career. We use writing to communicate our findings and share them with the world. If one doesn’t ...will be difficult or (more likely) impossible to find; therefore all the time spent collecting it will... course of the class, the moderator discussed individual sections of a paper, the abstract, figures, introduction...class, we met as a group for sessions covering individual portions of the manuscript. Stephen Blanke, Beronda...generating a quilt with your lab mates, you add the individual figures and results to make the final product...
Showing: 421 - 440 of 878 results