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We narrowed to 677 results for: Mos

Showing: 421 - 440 of 677 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...than regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated...antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ... will be represented through FSC-A vs. SSC-A, so most people use that setting. Figure 2: As the...the two cell types (likely B cells and others). Almost no cells stain positively for both markers. When...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...premature aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors...pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1...

  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...

  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...

  11. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...research is needed to determine which recA- strains are most effective at reducing multimerization and define...of Multimeric Forms of Circular Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128...

  12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...independent core facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas of the membrane... x-ray film or with specific imaging devices. In most cases, the primary antibody, which binds the protein...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...

  16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... is a powerful experimental strategy, one of the most common concerns we get here at Addgene about this...-homologous pairs) (Figure 2.). They found that almost all off-target expression can be attributed to ...

  17. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...Using a DNA template for the RNAi trigger is the most prevalent method due to ease of use and capacity...actively silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly ...

  18. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...expression in the retina and in parts of the brain, but almost none in the liver (A). In general, RNA levels tend...analyze the resulting data, so it may not be the most accessible option for many laboratories. Additionally...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...recognize the same epitope of the target protein. Most commonly these antibodies are produced from hybridomas...fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors...

  20. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...
Showing: 421 - 440 of 677 results