We narrowed to 457 results for: PAC

1. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ... is the transferable skills that create a full package and add an extra shine to your work. Knowing your...

2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...gene function while using fewer sgRNAs. These new compact libraries will allow scientists to conduct genome-wide...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...affinity for endogenous molecular targets such as paclitaxel. Unlike other systems, no genetically encoded...

4. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ...containing a phage origin of replication that can be packaged into replication-incompetent phage particles using...

5. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ...decrease. Furthermore, the size of the parts also impacts ligation efficiency, as concentration does not ...

6. CRISPR 101: Any Base Transversion Editors

   Type

   Blog Post

   Published

   April 1, 2025, 1:15 p.m.

   ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...

7. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   Published

   May 21, 2024, 1:30 p.m.

   ...candidacy, beyond what is on your resume. It is a space for you to explain how your skills and experience...

8. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...

9. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These are transfected into target cells...

10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type

    Blog Post

    Published

    Nov. 15, 2017, 3 p.m.

    ... of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...

11. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...and can include the pressure to publish in high-impact journals, and the requirement for a high grant ...

12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...high-resolution map of early embryogenesis in time and space, and was also used to precisely characterize developmental...

14. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...ready-to-use AAV from Addgene or check out protocols for packaging your own virus ...

15. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...into mainstream use, they will have a much greater impact on the life expectancy and overall survival of ...

16. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative, monogenic...

17. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

18. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...controls only address one type of error that can impact your western blot, and depending too much on normalization...

19. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...research will advance the field, create a new field, impact disease treatment, etc., again in clear terms. ...

20. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov ...