We narrowed to 452 results for: PAN

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent indicators for Ca2...

2. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ... interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning them sequentially...

3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ... if you are working for a large institution or company, site licenses might be available as well. In most...

4. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...party. Additionally, interest groups are often pandered to when strong or overlooked when small, but data...

5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined Mutagenesis.” Ed. D. Dafydd Jones...

6. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...advances in axolotl genome sequencing have vastly expanded axolotl research. One of the most significant ...

7. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   Published

   March 28, 2024, 1:15 p.m.

   ...integrin antibodies are the first deposit from an expanding collection of readily accessible recombinant antibodies...

8. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews sections...

9. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...conflict is actually critical in the business world. Companies and organizations that avoid all conflict can ...

10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...Chem Biol 27, 52-63 (2015). Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared...

11. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ... master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself...

12. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ...from academia altogether and instead started a company around his own research. He was clear on his science...

13. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...of Bacterial Expression Systems. Check out our companion post describing protein expression strains, where...

14. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute...

15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services...

16. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    Published

    Feb. 8, 2024, 2:15 p.m.

    ... are less compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you...

17. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ...

18. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    Published

    Dec. 20, 2022, 2:15 p.m.

    ...publication at Addgene. Riabinina, et al. “Improved and expanded Q-system reagents for genetic manipulations.” ...

19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...

20. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ... digestion varies for different enzymes. Many companies now sell fast digest enzymes that can digest large...