We narrowed to 447 results for: PAN

1. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews sections...

2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ... if you are working for a large institution or company, site licenses might be available as well. In most...

3. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ... interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning them sequentially...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined Mutagenesis.” Ed. D. Dafydd Jones...

5. 10 Steps to a Perfect Science Talk

   Type

   Blog Post

   Published

   Aug. 23, 2016, 2:30 p.m.

   ... topics that are unfamiliar to you because the company you are visiting works on them. Of course, if some...

6. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...In cases where the functionally important regions span several kb (or are GC-rich and therefore difficult...

7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ... master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself...

8. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...from academia altogether and instead started a company around his own research. He was clear on his science...

9. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...of Bacterial Expression Systems. Check out our companion post describing protein expression strains, where...

10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...

11. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    Published

    Feb. 8, 2024, 2:15 p.m.

    ... are less compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you...

12. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ...

13. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    Published

    Dec. 20, 2022, 2:15 p.m.

    ...publication at Addgene. Riabinina, et al. “Improved and expanded Q-system reagents for genetic manipulations.” ...

14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services...

15. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute...

16. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ... digestion varies for different enzymes. Many companies now sell fast digest enzymes that can digest large...

17. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...transposon in vertebrates. It was discovered in Japanese medaka fish because its insertion into the fish...

18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...assay kits are available from different biotech companies which are enzyme-based mycoplasma detection kits...

19. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 ...

20. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...their degradation within labelled cells limits the timespan available for tracking certain cell types. Another...