We narrowed to 499 results for: SHA

1. Antibodies 101: Flow Compensation

   Type

   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...those markers are identical and completely indistinguishable. This tool is of particular value when designing...

3. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...

4. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...do we make sure to catch all of these potential mishaps before distributing the vectors? Viral genome sequencing...

5. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully...

6. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ... in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since ...

7. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   Published

   July 12, 2018, 1:09 p.m.

   ...the effects of CO2 on sample pH Cell morphology: Shape and size matter You should have a light microscope...

8. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...through experience and from my mistakes. Here I’ll share what I wish I knew when I first became a manager...

9. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   Published

   July 2, 2020, 12:40 p.m.

   ..., Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture of Chromatin Interactions...

10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...the common antibody is heftier (~150 kDa) and Y-shaped because it contains two large heavy chains and ...

11. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...both sides of the postdoc experience, I’m going to share what I’ve learned and hopefully make your decision...

12. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering via...

13. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

14. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...therapeutically due to their unusually “smooth” shape and lack of “druggable” domains. In other words,...

15. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...sequencing results or comments on our suggestions? Share your thoughts here to help other labs speed up their...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with Endy more ...

17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

18. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ... actin filaments or microtubules. This issue is shared by other SRM techniques and can be alleviated by...

19. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...transparent about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his...

20. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...A few months ago, we shared an introduction to immunofluorescence (IF) - a common method for visualizing...