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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 844 results for: STI

Showing: 421 - 440 of 844 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...with a different editing window than existing CGBEs. This distinct approach means researchers can now select...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...other hosts. For example, the pJD4 plasmid harbors distinct origins (ori1, ori2, ori3) and replication initiation...these plasmids are compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and ... P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...methods measure different characteristics of the solution. These characteristics give us information on ... tries to get the other to guess a category by listing off things that fall into that category? Okay, ...do our best to send you a particular lot (if we still have it available). References 1. Fagone, Paolo...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and... University and former researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro....2014.09.039 Wong N, Liu W, Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9...

  5. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...customer support, but there are eight other teams who still retain a customer support element amongst their ...manager to explicitly assign the responsibility in question to a specific person or team. What Makes You ... will not be great at resolving conflicts.  Institutional Knowledge: Of the tools I’m mentioning here,...have used your empathy, listening skills, and institutional knowledge to resolve conflicts. Conflict in ...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...cellular world. The technique allows you to ask questions like: “Where does my protein of interest live ...corresponding secondary antibodies conjugated to distinct fluorophores to visualize multiple targets at ...Even with all of the resources available, it may still be difficult to find an antibody in which you are...not only answers to your most pressing research questions, but also stunning images (am I the only one with...

  7. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...of the world functions. Experiments come next, testing the correctness of the hypothesis. The results ...Environmental Protection Agency, and the National Institutes of Health endanger scientists’ ability to uphold...and inclusion through funding and at their own institutions, while many also seek to honor and credit women... funding is required. This is by no means an exhaustive list and every marcher is not necessarily marching...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...something that has fallen out of fashion but is still useful and most importantly, effective. In the research...silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes or to knock down ...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...valuable tool for your research arsenal, and there is still a dedicated field of study for improving these tools...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...membrane to enter cells. Depending on the research question and downstream application, one or several of ...are protected. Amine-binding viability dyes can still bind free amines on the cell surface, which yields...As a result, the largely negative live cells can still be easily gated on during the analysis. Conclusion...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly...events on its activation loop, but can be made constitutively active through phosphomimetic mutation of these...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. She is particularly...

  11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...infection, including both common infections and opportunistic infections not usually seen in healthy individuals...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...undigested DNA on a gel as a control when performing a diagnostic digest is a simple but effective method for detecting...assembly from short reads (<300 bp) which cannot distinguish between large repeat sequences within a plasmid...could try linearizing the plasmid via restriction digestion and then run the linearized plasmid on an agarose...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...you should investigate your options and weigh the potential benefits and costs before investing in one approach...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...multiple proteins but label each separately before reconstituting, choosing your colors depending on the needs...

  14. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...the endogenous RecBCD and SbcCD nucleases from digesting linear DNA introduced into the E. coli. Exo: Exo...spontaneous mutations that occur when Red is constitutively expressed. Which recombination system you use...interested in science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced...1.16.39. PubMed PMID: 24733238. 6. National Cancer Institute Recombineering Pages. Additional Resources on ...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...constructs (Moore et al., 2016). The variety of constitutive and inducible promoters, linkers, and tags makes...basic biology of and gene regulation in the opportunistic pathogen Acinetobacter baumannii (Brychcy et...delivery. While Fragmid developers were specifically testing CRISPR tools, it should be easy enough for researchers...tools and reagents already in place. MoClo is a fantastic way to save time and money on studies that require...

  16. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ... name! We thank you for your participation in suggesting names and voting in our poll. Now, we'd like ...didn’t have a name. Last month, we collected suggestions for names and we’ve recently chosen four names... BosLab, a community lab in Cambridge, MA, for hosting our agar art fun!...

  17. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...significantly reduced posting on November 20, 2023, and as of today, will stop posting, monitoring, and resharing...with active scientific communities and welcome suggestions in the comments.  X has moved significantly ...

  18. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...holidays may be over, but signs of holiday spirit still linger (despite the unseasonably warm weather we...the winning decorations for Deck The Lab. These festive, living artworks come from the Molecular Cytology... December 3, 2019 Hope you’ve enjoyed the festivities in the last month!  ...

  19. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...Christmas! created by the Moore lab at the Van Andel Institute. Krampus was created by Nate Levine.             ...using pipette boxes, wire, cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates...our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi...

  20. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was ...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024... can search for plasmids from a publication by pasting the paper's title into the Addgene website search...
Showing: 421 - 440 of 844 results