...membrane to enter cells. Depending on the research question and downstream application, one or several of ...are protected. Amine-binding viability dyes can still bind free amines on the cell surface, which yields...As a result, the largely negative live cells can still be easily gated on during the analysis. Conclusion...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

...Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly...events on its activation loop, but can be made constitutively active through phosphomimetic mutation of these...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. She is particularly...

...infection, including both common infections and opportunistic infections not usually seen in healthy individuals...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...

...undigested DNA on a gel as a control when performing a diagnostic digest is a simple but effective method for detecting...assembly from short reads (<300 bp) which cannot distinguish between large repeat sequences within a plasmid...could try linearizing the plasmid via restriction digestion and then run the linearized plasmid on an agarose...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286...

...the endogenous RecBCD and SbcCD nucleases from digesting linear DNA introduced into the E. coli. Exo: Exo...spontaneous mutations that occur when Red is constitutively expressed. Which recombination system you use...interested in science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced...1.16.39. PubMed PMID: 24733238. 6. National Cancer Institute Recombineering Pages. Additional Resources on ...

...you should investigate your options and weigh the potential benefits and costs before investing in one approach...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...multiple proteins but label each separately before reconstituting, choosing your colors depending on the needs...

... name! We thank you for your participation in suggesting names and voting in our poll. Now, we'd like ...didn’t have a name. Last month, we collected suggestions for names and we’ve recently chosen four names... BosLab, a community lab in Cambridge, MA, for hosting our agar art fun!...

...constructs (Moore et al., 2016). The variety of constitutive and inducible promoters, linkers, and tags makes...basic biology of and gene regulation in the opportunistic pathogen Acinetobacter baumannii (Brychcy et...delivery. While Fragmid developers were specifically testing CRISPR tools, it should be easy enough for researchers...tools and reagents already in place. MoClo is a fantastic way to save time and money on studies that require...

...significantly reduced posting on November 20, 2023, and as of today, will stop posting, monitoring, and resharing...with active scientific communities and welcome suggestions in the comments.  X has moved significantly ...

...holidays may be over, but signs of holiday spirit still linger (despite the unseasonably warm weather we...the winning decorations for Deck The Lab. These festive, living artworks come from the Molecular Cytology... December 3, 2019 Hope you’ve enjoyed the festivities in the last month!  ...

...Christmas! created by the Moore lab at the Van Andel Institute. Krampus was created by Nate Levine.             ...using pipette boxes, wire, cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates...our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi...

...contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was ...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024... can search for plasmids from a publication by pasting the paper's title into the Addgene website search...

...scientific support team, to tackle any experimental questions you may have. Our Director of Content, Brook, ...specialist (you'll know me by my Tweeting — er, posting!) and our favorite content strategist, Jo. While...each equally excited to talk to you, answer any questions, and get feedback on how we can be more helpful...

...your research and career goals, or to answer any questions you might have about Addgene. The page is divided...continuously updating this page with new and interesting content. Wish we had a video on something that...that you don’t see here? Give us suggestions in the comments section below! Additional Resources on the...

...finishing answering all submitted questions via blog post, which we will be posting in the next few weeks.)  Finally...diving into the ins and outs, tackling complex questions, and giving tips for consistently generating solid...

...pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February 13, 2021 Westenberg...chromoproteins in E. coli would make it easy to distinguish colonies from one another in the challenge. To...inoculated over a quadrant of an agar plate. Then, a stick is dragged through the first streak and spread over...

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...applying for industry jobs and deciphering job listings. Mobility between different positions at Novartis... project and scientific teams at the Novartis Institutes for BioMedical Research to develop novel biologics...

...academic institutions can afford to hire. It will be difficult for some of these institutions to manage... through your PhDs. Of course there were many fantastic posts in October. Click on the links below to ...

...holidays may be over, but signs of holiday spirit still linger on around the Addgene office as we peruse...ChristmasScene #womenwhoscience #scigirls #wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily...scene.  Got our @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz...

...information and has data on the date, material, requesting PI & organization, and country. This information...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...sharing of aggregated data only, information on requesting PI(s) & organization(s) is not shown on the dashboard...