We narrowed to 453 results for: Spr

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...others at the Sainsbury are working on a lot of CRISPR tools for editing plant genomes. We will definitely...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...Analysis of Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org...

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...Immunofluorescence Staining. Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

4. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...

5. A Guide to Starting Your Own Journal Club

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   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ... a week for several months at a time during the spring and fall semesters. Our scheduling was in part ...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...

7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...

8. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

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   Oct. 6, 2015, 2:30 p.m.

   ...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...

9. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...something fun is going on and to get there fast! Spread word of your plans by email, gong, evite, posters...

10. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...

11. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...

12. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...

13. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ... density of cells, while contour lines that are spread apart show a low density of cells (Figure 7).  ...

14. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

15. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...

16. Getting the Most from Your Lentiviral Transduction

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    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...

17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...

18. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...collaboration and the exchange of ideas. The rapid spread of optogenetics throughout the neuroscience field...

19. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they...

20. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ...