We narrowed to 546 results for: TIL;
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  TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....directly alongside the preprint while the work is still in progress or awaiting a final publication in a...
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Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....trendy as that may sound. Validated antibodies are still an excellent option for many applications, and frankly... - 
  
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....Germany. She presented a novel technology, back then still unpublished, that allowed the visualization of specific... - 
  
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) since 2005... - 
  
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....what if you just want to change protein levels? Until recently, no such tool existed for rapid, customizable... - 
  
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....2018). Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents... - 
  
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m.... (re-ligated empty vectors, for instance) would still express ccdB and therefore would die. This procedure... - 
  
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....informed discussion, debate, and data collection still remain: What are the current numbers of trainees... - 
  
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such that custom... - 
  
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....to work with than regulatory proteins. There are still things we haven’t solved that stop it from being... - 
  
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is ... - 
  
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....“flanked by loxP”), will permit gene expression until Cre is present, at which time the gene will be disrupted... - 
  
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction. Both... - 
  
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....they retain their intracellular interactions and still set off the same pathways, giving rise to the same... - 
  
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....cycle once inside the host. While the virus can still enter (and thus infect) cells, this safety feature... - 
  
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans." ... - 
  
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on... - 
  
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering... - 
  
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... - 
  
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....you can make big changes in your career path and still find comfort and happiness there. Read all of the...