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...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing...innovation.  In response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a...

...Chapter 6: The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer...and training the next generation of science communicators. In this chapter we learn about some of their...social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

...molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients...gene results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere...other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR to disrupt...Peritoneal Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and ...systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling pathways for their ...

...Your Insert and Vector by Gel Purification     Now that you’ve cut your insert and vector, unfortunately...unsuccessful 1), in a vector alone plate with many more colonies than the vector + insert plate (unsuccessful..., if you were cloning a gene into an expression vector, you would want the start of the gene to end up... ligation step. 3. Ligate Your Insert into Your Vector In the ligation step, you mix your purified, cut...Resources on Addgene.org Find the Perfect Empty Vector for Your Cloning Experiments Browse Our Molecular...

...Mary O’Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders in the...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... of the paper. They should be able to tell your story for you and they should also be easily understood...

...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...materials produced from your collaboration is a key factor in maintaining the group buy-in and morale. Especially...frequently the entire group will meet. You and your collaborators should then hold each other accountable to these...and circumstances permit, think about having collaborators spend time with each other in person. One possibility...workshops are another great place to meet up with collaborators. Being able to present data from multiple aspects...

...course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing... sure what to expect in terms of interest, but visitors to the poster were impressed to see how the numbers...

... work with a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager...(i.e. enough pay and benefits to live on), the factors that contribute to job satisfaction are a bit surprising...studies overwhelmingly agree that these are the factors that most contribute to job satisfaction: A feeling...Availability of superiors for consultation, advice and brainstorming. Employees must understand the part they play...team miserable.   A good manager keeps these factors in mind at all times when making decisions, assignments...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...

...ideas. What mentors are available for guidance, intelligent discussion, and/or brainstorming sessions to... your interests. We've outlined some important factors to consider and summarize helpful advice from the...Tips to Achieve Success in Academia". Important factors to consider when choosing a research project After...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...patient, being selective, and talking to peers and mentors will help elucidate which project is right for ...

...cytosine base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

...CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have ...

...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844 . https...true" %}{% raw %}"Browse Empty gRNA Expression Vectors"{% endraw %}{% end_module_attribute %}{% module_attribute...href":"https://www.addgene.org/crispr/empty-grna-vectors/","type":"EXTERNAL"},"open_in_new_tab":true,"no_follow... are even more exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of...breaks (Rees et al., 2018). For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window... guest blogger John Doench! John Doench is the Director of R&D in the Genetic Perturbation Platform at...

... or LinkedIn to learn more about their career trajectory. If there’s one thing everyone loves, it’s talking...can manifest via research and data gathering, regulatory efforts, policy implementation, program management...company who decides how they are going pursue regulatory approval for a new product or a well-endowed ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...example proposals and consider applying for their accelerator for some hands-on guidance.   Check out my webinar...careers, there are many ways to enter this career trajectory. One common route of entry is through a science...working in student government, and teaching and mentoring others (see more in Figure 2). Starting early ...

...Addgene depositors have made it easier to track translation in two different ways: by monitoring the first...complexes. Recent work has adapted this technique to monitor local translation and determine if proteins are...fusions to their reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. ...view for constant tracking. You can then directly monitor multiple rounds of translation from this single... Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate...

...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with...easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated... your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally, while...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...Page Check Out Our CRISPR Guide Pages Find Viral Vectors ...

...Research Campus to give researchers the ability to monitor glutamate release from neurons and other brain ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein...is also available in several ready-to-use viral vectors.    References and Resources Additional Resources... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

...expression of the protein. However, expression of receptors may not need to be as high to achieve an observable...titer may not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins...may not be necessary to concentrate this type of vector to such a high titer when looking for effective... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles... advances in recombinant adeno-associated virus vector production." Kidney international 61.1 (2002): ...Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS ...

...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...