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We narrowed to 863 results for: TOR;

Showing: 421 - 440 of 863 results

  1. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...materials produced from your collaboration is a key factor in maintaining the group buy-in and morale. Especially...frequently the entire group will meet. You and your collaborators should then hold each other accountable to these...and circumstances permit, think about having collaborators spend time with each other in person. One possibility...workshops are another great place to meet up with collaborators. Being able to present data from multiple aspects...

  2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing... sure what to expect in terms of interest, but visitors to the poster were impressed to see how the numbers...

  3. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... work with a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager...(i.e. enough pay and benefits to live on), the factors that contribute to job satisfaction are a bit surprising...studies overwhelmingly agree that these are the factors that most contribute to job satisfaction: A feeling...Availability of superiors for consultation, advice and brainstorming. Employees must understand the part they play...team miserable.   A good manager keeps these factors in mind at all times when making decisions, assignments...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...

  4. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...ideas. What mentors are available for guidance, intelligent discussion, and/or brainstorming sessions to... your interests. We've outlined some important factors to consider and summarize helpful advice from the...Tips to Achieve Success in Academia". Important factors to consider when choosing a research project After...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...patient, being selective, and talking to peers and mentors will help elucidate which project is right for ...

  5. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...cytosine base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

  6. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have ...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... or LinkedIn to learn more about their career trajectory. If there’s one thing everyone loves, it’s talking...can manifest via research and data gathering, regulatory efforts, policy implementation, program management...company who decides how they are going pursue regulatory approval for a new product or a well-endowed ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...example proposals and consider applying for their accelerator for some hands-on guidance.   Check out my webinar...careers, there are many ways to enter this career trajectory. One common route of entry is through a science...working in student government, and teaching and mentoring others (see more in Figure 2). Starting early ...

  8. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...expression of the protein. However, expression of receptors may not need to be as high to achieve an observable...titer may not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins...may not be necessary to concentrate this type of vector to such a high titer when looking for effective... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles... advances in recombinant adeno-associated virus vector production." Kidney international 61.1 (2002): ...Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS ...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with...easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated... your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally, while...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...Page Check Out Our CRISPR Guide Pages Find Viral Vectors ...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Addgene depositors have made it easier to track translation in two different ways: by monitoring the first...complexes. Recent work has adapted this technique to monitor local translation and determine if proteins are...fusions to their reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. ...view for constant tracking. You can then directly monitor multiple rounds of translation from this single... Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate...

  12. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...Research Campus to give researchers the ability to monitor glutamate release from neurons and other brain ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein...is also available in several ready-to-use viral vectors.    References and Resources Additional Resources... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  13. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

  14. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...

  15. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...resources at Addgene. His background in history and specifically the history of RNAi left him a bit skeptical...to the Advisory Board. John Doench is Associate Director of the Genetic Perturbation Platform at the Broad...Listen to the interview here! Doench majored in history during his undergraduate work at Hamilton College...targets and what confers specificity in the miRNA regulatory process. This early research is still relevant...function really…. Addgene’s role as a central repository for what is useful and what was used once and...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...this list, that does not necessarily mean the repository does not have it. That said, if you know of a... don’t hesitate to start the process! Addgene depositors who study chromatin The PIs linked below are ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin Cheryl Arrowsmith James...SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek...Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors or not, your time is valuable ...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...textbook, a laboratory kit, and support during the academic year from remote scientist mentors and the BioBuilderClub...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days in the classroom...meetings and newsletters, pairs teams with STEM mentors, and provides a nationally recognized platform ...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education...

  18. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...define what type of factor you are looking for and what process or outcome that factor functions in. The...there is removal of known factors (such as a genes) and the loss of those factors is assessed. The two most... committed to the daunting task of finding new factors in your biological system of interest, the so-called...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a set of either...by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and Cas12a, ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene...packaged in AAV. A dCasMini-mediated adenine base editor allows for base editing, while gene editing can...

  20. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing various...expression using either TEF1 or GAL1 promoters. These vectors are intended for the high level expression and ...spike protein) cloned into a CMV driven expression vector are also available. These contain no tags and are... genes in E. coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs ...status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...
Showing: 421 - 440 of 863 results