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Showing: 421 - 440 of 705 results

  1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...each, thereby achieving the coveted “blue flame” status. We’re proud to honor the creativity, passion, ...that we cover all of the shipping logistics and facilitate all material transfer agreements, thereby freeing...

  2. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  3. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that...

  4. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...preparing for my first official professional presentation.  My first time in the lab! I got to assist...should have been nervous), but I wasn’t. The presentation went along smoothly and was followed with great...

  5. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the... the “rabbit hole” of related cell lines when annotating a cell line Community submissions via email: ...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents, and...

  7. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  10. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

  11. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  16. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

  17. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ...

  18. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...extract.    Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

  20. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal...
Showing: 421 - 440 of 705 results