We narrowed to 696 results for: Tec

1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...containing lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin...

2. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

3. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

   Type

   Blog Post

   Published

   May 25, 2021, 2:29 p.m.

   ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating...

4. Antibodies 101: Chimeric Antibodies

   Type

   Blog Post

   Published

   April 25, 2023, 1:15 p.m.

   ...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B...

5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts  can be easily customized to detect other sequences of interest.   While the VGS process...

6. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   Published

   Oct. 10, 2019, 12:41 p.m.

   ... genetically encoded antibody based probe that detects the widely used HA epitope in diverse cellular ...dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and scaffolds...

7. Single-cell tracking of lineage and identity with CellTag

   Type

   Blog Post

   Published

   May 9, 2019, 1:15 p.m.

   ...desired identity. The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now...multi-generational cell lineage trees. CellTags were detected over a 10-week period. Their expression decreased...

8. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

   Type

   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid...

9. The Strength of Story Telling

   Type

   Blog Post

   Published

   Dec. 19, 2017, 2 p.m.

   ...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay...

10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare...

11. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    Published

    Aug. 21, 2014, 3:15 p.m.

    ...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

12. Extracurricular Activities for a Strong Science Career Path

    Type

    Blog Post

    Published

    Jan. 21, 2014, 3:08 p.m.

    ...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

13. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    Published

    Aug. 4, 2020, 1:15 p.m.

    ...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

14. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type

    Blog Post

    Published

    June 30, 2020, 1:15 p.m.

    ...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some...

15. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID...

16. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements...

17. Addgene Depositors Get More Citations

    Type

    Blog Post

    Published

    April 20, 2017, 2:30 p.m.

    ...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ...

18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    Published

    April 7, 2022, 1:15 p.m.

    ...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

19. With Our New Viral Service, We're Taking CRISPR Further!

    Type

    Blog Post

    Published

    Dec. 6, 2016, 3:30 p.m.

    ...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

20. Hot Plasmids - January 2023

    Type

    Blog Post

    Published

    Jan. 31, 2023, 2:15 p.m.

    ...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...