We narrowed to 693 results for: Tec
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TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....bacterial strains, such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI...Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....containing lentiviral vectors the iBARs can be detected by next generation sequencing. In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts can be easily customized to detect other sequences of interest. While the VGS process... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m.... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions Market analyses... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m.... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m.... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI. Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m.... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...