Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 907 results for: addgene

Clear keywords
Showing: 421 - 440 of 907 results

  1. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

  2. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References...

  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...

  5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome... troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our...the correct clone. Additional resources on the Addgene blog Get all the plasmid basics in our Plasmids...molecular biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the ...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...resources on addgene.org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101...

  8. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...

  10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

  11. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

  12. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...science communication Resources on Addgene.org Learn how Addgene engages with the community ...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

  14. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts...

  15. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...

  16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Deliver...Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell Lines with Lentivirus...

  17. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  18. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a... the Cpf1 multiplexing plasmids available from Addgene. If you have any questions or thoughts about Cpf1...Central PMCID: 4638220. Additional Resources on the Addgene Blog Cpf1: A New Tool for CRISPR Genome Editing...in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing Resources Find...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...PubMed PMID: 19185711. Additional Resources on the Addgene Blog Beware of Fluorescent Protein Oligomerization...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes...bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More about Homology Directed Repair... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...
Showing: 421 - 440 of 907 results