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TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m....Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...
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5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m.... for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline ...CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and more References... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects. Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome... troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our...the correct clone. Additional resources on the Addgene blog Get all the plasmid basics in our Plasmids...molecular biology protocols and tips Resources on Addgene.org Learn more about Sanger sequencing Read the ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....desired genomic edit. Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells. Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m.... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....science communication Resources on Addgene.org Learn how Addgene engages with the community ...BioTechniques. Additional resources on the Addgene blog Find all of our science communication posts... -
Making Career Development an Integral Part of the Science Training System
TypeBlog PostPublishedFeb. 25, 2021, 2:15 p.m.... Additional resources on the Addgene blog Get started with Addgene's science career resources Browse... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....will make those new plasmids available through Addgene, and they hope others will do the same. “This isn...10.1186/1754-1611-8-7. PubMed. Related Resources Addgene's Synthetic Biology Overview More Synbio Blog Posts... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Deliver...Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell Lines with Lentivirus... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440. Additional resources on the Addgene blog Read all of our viral vectors blog posts ... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...References and Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies...resources on addgene.org Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose. Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...