We narrowed to 529 results for: arl
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TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....increase your success rate. 1. Submit your grant early! First off, and perhaps the most important bit of...
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Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m..... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....additional references are provided at the end. Nearly all common bacterial expression plasmids use Rho-independent... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....serial dilutions of a viral prep. This method is particularly useful when vectors carrying fluorescent markers... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....Lars O., et al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....would need much more guidance, direction, and a clearly laid out plan of action, others would act more ... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m.... this question at my workshops, I get nods from nearly every participant. Robert Bramson, author “Coping... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....cells are a great option for viral production, particularly for labs that already do mammalian cell culture... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target of intense... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....with childcare timing pickup. Whoever takes the earlier slot agrees to pick up both children while the ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m.... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... her PhD from Northeastern University and is particularly interested in cell signaling and communication... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....researchers often use the Cre/Lox system described earlier. In this case, you design your targeting vector... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....performed additional efficiency measurements using similarly sized Lambda amplicon inserts to control for any... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m.... protein structure in a flat 2-D images. In the early days of structural biology, Byron Rubin, a crystallographer... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....marker can be removed by Cre-mediated excision for scarless tag insertion. In addition to the obvious advantages...