We narrowed to 512 results for: ato

1. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical...

3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently edited the fetal...

4. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5...

5. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ... in genetics research, their applications and regulatory policies; keeping tabs on the conversation about...

6. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...Destination vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression...

8. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...

9. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...

11. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has been shown to increase...

12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...

13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...

14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...trying to explore the links between genetics and anatomy, which are not yet fully understood. We attempt...

15. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...

16. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

17. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

18. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

19. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...