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...important to understand that these platforms, or pieces of a platform, that are now available exist within...’s only the promises and associated sequence information. Addgene has partnered with the BioBricks Foundation... good ways of converting typically invisible information into actionable signals. Let's say I wanted to...

...have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...when the kits buffers ran out. Homemade buffers performed the same as those provided with the kit. Although...the results of transfection assays. Go forth and perform your DNA extractions without kits! Regardless of...

...all over the world looking for some additional information or clarification on these vectors. Read on to... for packaging (that's gag, pol, and env), the isoforms of these proteins, as well as the viral long terminal...shown to repress expression of HIV-1 LTRs (more information is available here). 2nd vs. 3rd Generation Lentiviral...with respect to the specific experiments being performed and you should understand the guidelines for using...

... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...your Sanger sequencing files and provide basic information such as your sample names and guide sequences...scalability, the ICE software has two analysis formats: sample by sample analysis, which can compare up...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking...

...protocol(s). Is there enough information there for you to be independent performing the technique you trained...benefits: you may learn faster and retain more information plus your mentor will get you up to speed easier...you need from them, and you can understand the information in your training. Bench mentor-mentee relationships...

...develop a successful, reproducible assay. That information can make you a much stronger candidate!  Additional...via their papers and/or website and use that information to explain why you are interested in working ...of a cover letter Cover letters follow the same format regardless of whether you’re in academia, industry... preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated...

...the BP and/or LR reactions are performed, the next step is to transform competent E. coli cells and select...our recent Plasmids 101 Post on CcdB for more information. How to clone using Gateway technology To better...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for...

..., our volunteers have been helping to gather information for our FLSA and postdocs resource on what institutions...postdoctoral salaries are changing. Transparent salary information for postdoctoral positions is very hard to find... may face greater challenges. Current salary information that we have been able to find suggests that ...Please also feel free to contact us if you have information that you think would help our resource. In the...

...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...selling yourself and really do just want to get information about yourself and your work out to other scientists...networking. I was an early adopter of the LinkedIn blog format and it is a great way to get people to resources...

... the reference section provide more in depth information. Technical replicates An example of a technical... to the consistency of the reagents and hands performing the experiment, not the reproducibility or consistency...independent aliquots of cells and plasmids, be performed on different days, etc, but outside constraints...incorporating additional positive and negative controls, performing multiple replicates, and ultimately getting interpretable...

...of off-target DNA editing and on-target indel formation. For the safety of KJ’s experimental treatment...cellular models of KJ’s mutation, SpG Cas9 + ABE8e performed the best when paired with the highest-efficiency...produced efficient editors, the Kleinstiver set performed best when paired with the V106W mutation (described... assessed for off-target risks, such a modular platform could accelerate the development of genetic therapies...

... virus directly using saliva without having to perform RNA extraction but that may work only if there ... Motif) for target cleavage. This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK...fluorescently labelled substrate, gets activated and performs non-specific collateral cleavage. This cleavage...emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses...

...mapping and modern sequencing Restriction mapping informs on DNA sequence through the use of restriction ...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...techniques still have useful applications and have informed many current techniques. For example, restriction...sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID:  25743089 ....

...are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...For a more comprehensive list and additional information, visit OpenWetWare's E.coli genotype resource...

...several brain regions where other serotypes may perform better (3). Brain Region  Optimal Serotype ...expressed that anatomical assessments can be easily performed too. While I implied that axoplasmic transport...vectors that will have substantially improved performance profiles and should be suitable for genome editing...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual...expression in the biotech workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized.... “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer ...

...shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo...Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies...

...students transform them into different strains of E. coli,” says Westenberg. During the transformation experiment...genetic backgrounds of the host strains affect transformation efficiency.  Aside from the Streak Plate Challenge...

...of empirically derived and/or prior knowledge to inform on the qualities of what determines good sgRNA ...tools provide results to the user in different formats. This can have a large impact on downstream results...save”. Trends: Most tools provide tabular HTML formatted output. Surprisingly only 2 tools provide FASTA...idea of what to look out for. Top tools for bioinformaticians: For flexibility in your own analysis, you...

... ship individual plasmids as transformed bacteria in stab culture format. Our lab team creates stabs for... for each plasmid being shipped based on the information in our lab software, labeling stabs with barcodes...