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Showing: 421 - 440 of 756 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ..., our volunteers have been helping to gather information for our FLSA and postdocs resource on what institutions...postdoctoral salaries are changing. Transparent salary information for postdoctoral positions is very hard to find... may face greater challenges. Current salary information that we have been able to find suggests that ...Please also feel free to contact us if you have information that you think would help our resource. In the...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...selling yourself and really do just want to get information about yourself and your work out to other scientists...networking. I was an early adopter of the LinkedIn blog format and it is a great way to get people to resources...

  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...your Sanger sequencing files and provide basic information such as your sample names and guide sequences...scalability, the ICE software has two analysis formats: sample by sample analysis, which can compare up...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... the reference section provide more in depth information. Technical replicates An example of a technical... to the consistency of the reagents and hands performing the experiment, not the reproducibility or consistency...independent aliquots of cells and plasmids, be performed on different days, etc, but outside constraints...incorporating additional positive and negative controls, performing multiple replicates, and ultimately getting interpretable...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... virus directly using saliva without having to perform RNA extraction but that may work only if there ... Motif) for target cleavage. This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK...fluorescently labelled substrate, gets activated and performs non-specific collateral cleavage. This cleavage...emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses...

  6. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...mapping and modern sequencing Restriction mapping informs on DNA sequence through the use of restriction ...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...techniques still have useful applications and have informed many current techniques. For example, restriction...sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID:  25743089 ....

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...several brain regions where other serotypes may perform better (3). Brain Region  Optimal Serotype ...expressed that anatomical assessments can be easily performed too. While I implied that axoplasmic transport...vectors that will have substantially improved performance profiles and should be suitable for genome editing...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual...expression in the biotech workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized.... “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer ...

  9. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo...Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies...

  10. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...students transform them into different strains of E. coli,” says Westenberg. During the transformation experiment...genetic backgrounds of the host strains affect transformation efficiency.  Aside from the Streak Plate Challenge...

  11. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...of empirically derived and/or prior knowledge to inform on the qualities of what determines good sgRNA ...tools provide results to the user in different formats. This can have a large impact on downstream results...save”. Trends: Most tools provide tabular HTML formatted output. Surprisingly only 2 tools provide FASTA...idea of what to look out for. Top tools for bioinformaticians: For flexibility in your own analysis, you...

  12. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ... ship individual plasmids as transformed bacteria in stab culture format. Our lab team creates stabs for... for each plasmid being shipped based on the information in our lab software, labeling stabs with barcodes...

  13. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...out an updated AAV Data Hub to share curated information about AAV usage in research applications. Scientists...what challenges remain in materials sharing, information sharing, and research reproducibility while expanding... and distributing high-quality materials and information. As a scientist, I have seen both the potential...

  14. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...official document listing materials and pricing information that is sent from a buyer to a seller. However...your order online has some benefits. All of the information that is entered at checkout is used to generate... process, the Addgene Help Center is full of information about how to deposit plasmids, store your plasmids...

  15. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ... list of citing articles under the "Resource Information" heading in the right column of the plasmid page... the "# References" link under the "Resource Information" heading, you'll be directed to a page listing...also included a feature to help save you from performing any unnecessary cloning - the list of "Associated...

  16. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...your AAV plasmid of choice for more information on its performance.   Titers We expect titers for plasmids... and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend you...

  17. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...recommended reagents) along with information critical for the performance of a reagent, e.g., antigen retrieval...Knowledge-Base is an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX ...

  18. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...retrograde tracing: visualizing neurons that received information from a region. In the example shown in Figure...by sharing not only reagents, but also method information. To complement these efforts, we’re beginning...through our recently launched AAV Data Hub, an open platform where researchers using AAV from our viral service...

  19. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ... extracellular surface of the GPCR causes a conformational change in the GPCR, leading to the activation...that the potency and efficacy is typically non-uniform across the transducerome for a specific receptor-ligand...Strachan RT (2020) TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat ...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...equipment, or ensure that techniques are being performed the same way at different sites. I did a lab exchange...
Showing: 421 - 440 of 756 results