We narrowed to 823 results for: lars;

...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...

...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

.../phototoxicity Not susceptible Susceptible Subcellular imaging Improving Well-established High-throughput...bioluminescent reporter to be cloned and remains popular, other luciferases have been identified in the ...luciferase is measured via the light produced. A similar approach can be used to analyze cell signaling ...brings the two luciferase components together. A similar approach can test protein-protein interactions ...

...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics...Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...method, but can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with...leveraged the power of Golden Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo...101: Multiplex Expression of gRNAs Choosing a Molecular Cloning Technique  ...

...avenues for therapeutics that treat disease at the molecular level. For instance, if a disease is caused by...vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery vehicles...hemophilia B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable...on gene therapy for viral infections. She is particularly passionate about HIV cure research and science...

...skills Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association...provides a peak into their day-to-day business with popular work experiences of 3-10 days. And the Society ... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

... several reasons. Casɸ is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

... your interview The STAR interview method is a popular way to answer behavioral interview questions. STAR...key decision. Even deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

...and resources page Browse our molecular biology protocols Read our molecular biology reference ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...worm so check back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN...

...need to design and optimize a pegRNA for your particular edit. These tips are a great starting point: ...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22...

...edit due to toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature...with neuron bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly...