We narrowed to 486 results for: multi
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TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles, many...
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Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....meet with various professionals, and there were multiple avenues for professional development including... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....for transformants. It’s very important to select multiple T1 plants from each transformation. I like to ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....enough... If in the past you have tried to combine multiple terms in an Addgene plasmid search and found the... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....targeting a specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....certainly chapters that could warrant their own books. Multiple books have been written about CRISPR alone, and... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....you do need to work with dilute samples: Take multiple readings to average out noisy measurements. Be... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating adenovirus using... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....Introduction to Immunofluorescence Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity Reagents... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....measurement divided by the mean of all the replicates multiplied by 100. To calculate the %CV of the standard ... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m.... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....it is possible to measure the concentration of multiple targets simultaneously. For AAV titration, you... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....PMCID: PMC110254. 3. In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify the insert... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional plasmid ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....and PLoS One. Additionally, you are involved in multiple professional scientific societies. Why is being... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....and probably others) Neutral mutations A multitude of applications Due to its size and ease of use...