We narrowed to 526 results for: proc

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences 104:5163–...

2. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

3. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...enzyme recognition sites. As a result, the ligation process is close to 100% efficient. Another strength of...

4. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...

5. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

6. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...overhangs. Exploring the efficiency of these two processes will help us better understand where Cpf1 fits...

7. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

8. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

9. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...maintain your parental strains throughout this process. In the F1 and later generations, mice that are...

10. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting...

11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...activation across multiple loci was an arduous process. When using zinc finger proteins or TALE proteins...

12. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...While this option is attractive because the same procedure for a single 10 cm2 dish can be easily applied...

13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...lines and use these lines to study how immune processes and biological pathways are altered in PMM2-CDG...

14. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...

15. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...Schematic of the goldilocks antibody selection process The exposure time of the primary antibody compared...

16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...

17. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...to your parameters.   Kinetics How fast is the process you would like to monitor? Neurons, for example...

18. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...

19. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...have been cross-adsorbed. The cross-adsorption process filters out off-target antibodies increasing specificity...

20. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ... and as societal participants in the political process. My role at pgEd I have always been very interested...