We narrowed to 818 results for: tes

...is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences...molecular pathways, I found myself curious about other routes – In terms of both types and topics of work. A ...rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination to be ...

...of heredity were thought of as everything from parasites, to symbionts, to genes and the labels applied... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... that is probably the easiest path. Some institutes have structured visiting positions within defined ... any protocols the day before, etc.), take good notes, and ask questions if you don’t understand something...

...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...elevated at stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can...conditions better than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative...

...stood out from their initial testing. It outperformed other tRNA candidates (either from human or plant...muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells, including...

...measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of the buffer...-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments move ...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

...specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further differentiated into dentritic...decisions about in vivo application and clinical testing. This would pave the way for improved patient outcomes...Burns-Naas, L. A. (2015). Chapter 27—Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis...

...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...

...variety of research applications and particularly notes their ability to be precisely spatio-temporally ...down the HMO of interest, the lactose released activates expression of GFP from the lac operon. GFP expression...Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent Protein for Your Experiments Resources...

...CRISPR/Cas system, Cas9 is guided to its target sites with the aid of two RNAs: the crRNA which defines... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist of repeated...

... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

...plasmid, and can be assayed conveniently. A direct test for your gene of interest may be another method ...always mission critical to make sure the plasmid replicates in the cell, as many of these experiments are... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

... start, the team inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry...SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-SOS fusion protein...membrane-localized mCherry. With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (...

...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...lineage, and so much more! Libraries allow you to test thousands of elements simultaneously in your system...dose. Vaccine development strategies. Image courtesy of Gorry et. al, 2007.   Clinical applications...

...below). Plating the transformation on ampicillin plates containing X-gal allows you to identify cells that...that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase. Complementing...this deleted portion, for example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens...

...repeat region on its 3’ end. This repeat region associates with the trans-activating crRNA (tracrRNA) which...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications, proved more...

... both on and off target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison...Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the...

...can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al., 2018).  To create ...lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment...again that sgRNA structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They...

... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines, featuring both a general...