We narrowed to 707 results for: ust

...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...

...eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian ...are transformed to 100. This transformation (illustrated in the graph below) is performed because weak...

..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....

...to be sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all...problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize...

...which we will discuss later.       Standard curves must be included every time the assay is run, ideally...protocol is being followed.  Finally, standard curves must be validated for each run. To validate, include ...

...you would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day ...

...cells (Figure 1). The resulting HeLa cells show robust labeling, with strong expression and detection ...can be used to build these types of constructs robustly, quickly, and simply. We hope that you can similarly...

...to share it with others! After all, won't it work just as well for everyone else?  In truth, people are...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye...

... things contributed to the steadily increasing distrust the general public has in science and scientists...chapters in this section describe the challenges of trusting science in a “fake news and post-truth era,” the...

...tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding your sample...tricks for performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR...

...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018).... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

...tools designed to help reduce or eliminate bias, just like the ones made available through the work done...Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting ...

...Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...

...past 5-10 years, the Grants Profession has become just that, a profession. A grant professional works for... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

...sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein...which a good antibody has not been developed (and just because your protein has a commercially available...

...coding sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts...the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...

... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...

... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...

...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...

...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...