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...ImageJ plugins JACoP and Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software...select FPs for muli-color imaging. Furthermore, one must bear in mind that the resolution of light microscopy...

...like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we select...recombined cells. Recombination is a rare event, so you must select as opposed to screen for cells where recombination...

..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....

...to be sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all...problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize...

...you would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day ...

...which we will discuss later.       Standard curves must be included every time the assay is run, ideally...protocol is being followed.  Finally, standard curves must be validated for each run. To validate, include ...

... things contributed to the steadily increasing distrust the general public has in science and scientists...chapters in this section describe the challenges of trusting science in a “fake news and post-truth era,” the...

...tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding your sample...tricks for performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR...

...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018).... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

...tools designed to help reduce or eliminate bias, just like the ones made available through the work done...Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting ...

...to share it with others! After all, won't it work just as well for everyone else?  In truth, people are...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye...

...cells (Figure 1). The resulting HeLa cells show robust labeling, with strong expression and detection ...can be used to build these types of constructs robustly, quickly, and simply. We hope that you can similarly...

...Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...

...past 5-10 years, the Grants Profession has become just that, a profession. A grant professional works for... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

...sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein...which a good antibody has not been developed (and just because your protein has a commercially available...

...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...

...control by Addgene before being distributed to customers. Details about our production protocols, titering...Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references Resources at the...

...editing treatment would have to be individually customized to the patient's specific mutations. With a drag... Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang,...

...place people share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide... idea into a viable solution.  Anticipate Change Just because a process is working, doesn’t mean it will...

...affect binding the lysis buffer may need to be adjusted depending on the sample. It is important to remember...make this approach appealing, but the disadvantages must be carefully considered before using. For example...