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We narrowed to 707 results for: ust

Showing: 421 - 440 of 707 results
  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...
  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....
  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...which we will discuss later.       Standard curves must be included every time the assay is run, ideally...protocol is being followed.  Finally, standard curves must be validated for each run. To validate, include ...
  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...you would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day ...
  5. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...cells (Figure 1). The resulting HeLa cells show robust labeling, with strong expression and detection ...can be used to build these types of constructs robustly, quickly, and simply. We hope that you can similarly...
  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...to share it with others! After all, won't it work just as well for everyone else?  In truth, people are...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye...
  7. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... things contributed to the steadily increasing distrust the general public has in science and scientists...chapters in this section describe the challenges of trusting science in a “fake news and post-truth era,” the...
  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding your sample...tricks for performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR...
  9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018).... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...
  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...tools designed to help reduce or eliminate bias, just like the ones made available through the work done...Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting ...
  11. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...
  12. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...past 5-10 years, the Grants Profession has become just that, a profession. A grant professional works for... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...
  13. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein...which a good antibody has not been developed (and just because your protein has a commercially available...
  14. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...coding sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts...the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...
  15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...
  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...
  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...
  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...
Showing: 421 - 440 of 707 results