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We narrowed to 694 results for: ust

Showing: 421 - 440 of 694 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding your sample...tricks for performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...to share it with others! After all, won't it work just as well for everyone else?  In truth, people are...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...you would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day ...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...which we will discuss later.       Standard curves must be included every time the assay is run, ideally...protocol is being followed.  Finally, standard curves must be validated for each run. To validate, include ...

  5. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...cells (Figure 1). The resulting HeLa cells show robust labeling, with strong expression and detection ...can be used to build these types of constructs robustly, quickly, and simply. We hope that you can similarly...

  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...tools designed to help reduce or eliminate bias, just like the ones made available through the work done...Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting ...

  7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018).... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...

  9. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...past 5-10 years, the Grants Profession has become just that, a profession. A grant professional works for... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein...which a good antibody has not been developed (and just because your protein has a commercially available...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

  12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence... SpCas9, indicating that the variants are likely just as selective as wild-type SpCas9. These VQR, EQR...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag... Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang,...

  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...place people share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide... idea into a viable solution.  Anticipate Change Just because a process is working, doesn’t mean it will...

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...affect binding the lysis buffer may need to be adjusted depending on the sample. It is important to remember...make this approach appealing, but the disadvantages must be carefully considered before using. For example...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...

  18. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...control by Addgene before being distributed to customers. Details about our production protocols, titering...Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references Resources at the...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...
Showing: 421 - 440 of 694 results