We narrowed to 904 results for: CAN

...high accuracy. Conversely, you can only run one sample at a time, which can become costly and impractical...is generally cheaper if you have many samples and can output more data, and it typically results in high...structures, or repetitive regions. Short-read sequencing can also be time-consuming, depending on the library ... to hundreds of base pairs, long-read sequencing can read sequence lengths in the thousands or even millions...by the instrument changes. Base-calling software can then identify the nucleotide that passed through ...technology. Created with BioRender.com. Nanopore can provide longer read lengths of 10 kb to 100 kb (or... to sequence an entire plasmid in one read. Runs can be completed in anywhere from under an hour to 72...

...common activities can be done straight from the new search bar, but the menu just above it can help you quickly...make your experience on our website better so you can get the resources you need more easily.  Here’s ...if you’re in the mood to browse our catalog, you can use the links under the search box to go straight...   Figure 1: The new search bar.   Pro tip! You can use a manuscript title to search for plasmids from... our science options into a single place, so you can quickly navigate around the Addgene website without...

...- links to articles citing this plasmid. Now you can learn how a plasmid has been used by multiple labs...listing the articles that cite this plasmid. You can use the dropdown to increase the length of the list...list (purple oval in the screenshot below). You can also use the "Search Table" box at the upper right...list of citing articles. From the article list you can click on the PubMed link to find the article abstract... database - and we always appreciate any help we can get. If you know of an article that used a plasmid...plasmid from Addgene, we want to hear about it so we can link to it in the "Articles Citing This Plasmid" ...

...fluorophore can emit a limited number of photons before bleaching. The amount of information one can extract...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising...budget, which determines how long and precise we can track individual molecules. Poor photon budgets are...fluorescent proteins—even the thriftiest cell biologist can feel like a college student searching couch cushions...more photons from a sample. Chemical fluorophores can be substantially brighter and more photostable than...transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and then...1e) are inherently fluorogenic and several others can be made fluorogenic using environmentally sensitive...

... of a particular observation can be easily studied as each component can be examined independently. In...thus modeling genome replication. These products can be detected by Northern blot analysis. In addition...producing firefly luciferase. Firefly luciferase can readily be measured to quantify (indirectly) transcription...by the viral polymerase. Alternatively, the mRNA can be detected directly by Northern blot analysis or...In addition, experiments can be performed in the absence of some viral proteins (compared to viral infection...Therefore, the roles/functions of individual proteins can be studied independently of other viral proteins ...simplifying the interpretation of results. This system can be used to develop an understanding of and manipulate...

...secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity can appear reduced...they can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve...is limited in the number of specific targets you can label in a sample. Super-resolution microscopy (SRM...original size. Depending on the protocol, samples can be expanded to ~4-10x linearly! This expansion physically...said, the anchoring, digestion, and expansion steps can impact the integrity of different targets and labels..., et al. 2020). With any variation, your samples can tear or expand unevenly, introducing distortions ...expansion results in dimmer samples. Reduced signal can be caused by loss of protein during digestion, or...

...tissue-specific diseases, as you can target your library to a significant degree using different types of...       In vivo, in vitro, ex vivo CRISPR screens can be carried out using a few different methods — in...infected with the CRISPR library. In vivo screens can become costly, so having an efficient and effective...delivery method for in vivo CRISPR screens. AAV vectors can be made at high titers equal to those achieved with...affect which is chosen for certain experiments. AAV can be safely handled at BSL-1, while lentivirus requires...vectors being packaged using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at ... interest — for example, AAV8 for kidney cells — can help improve infection rates in your experiment. ...

...the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group of prey fusions can either be...experiments. False positives can occur even in small scale experiments, although these can often be accounted for...cellular compartment or growth stage). A prey library can either be applied to the bait in a single population...false negatives; up to 75% of known interactions can be missed by single-pass two-hybrid screening (6)...levels of bait and prey proteins - overexpression can lead to false interactions. Lowered expression levels...for problems with one or more of these parameters can lead to a high number of false negatives in Y2H screens...responsible for the modification in the assay host strain can help. In order to reduce the chance that the configuration...

...titers can drop 10-fold for every 2kb increase in insert size. To overcome this, lentivirus can be concentrated...popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system...competent lentivirus (RCL). Replication competence can arise from recombination events during viral production...fourth plasmid. The requirement for additional DNA can impact the transfection efficiency of the packaging...Requiring an additional plasmid for transfection can make these optimization experiments a bit more challenging...transfection conditions. As mentioned above, plasmid ratios can be adjusted to enhance titer. In addition the transfection...for an example of how changing transfection ratios can affect transfection efficiency. For some transgenes...

... primary cells, embryos, and animals. Nanoblades can also carry different types of CRISPR cargo including...Nanoblades then bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein...the two proteins, thus releasing Cas9. Nanoblades can also deliver Cas9 variants, such as a transcriptional...Gag-Cas9 fusion protein. A single Nanoblade particle can be loaded with 1-4 different gRNAs. Viral envelope...showing that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...carries a gRNA, Cas9, and a HDR donor template DNA can be generated by incubating Nanoblade particles with...zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades, however...