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We narrowed to 928 results for: Gene

Showing: 221 - 230 of 928 results

  1. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...within the gene/insert, this information can be found here. There may be more than one gene inserted into...can easily clone my gene into it? Your best bet is the Plasmid Map: We auto-generate a plasmid map based... deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This describes...the backbone, and provides information about this gene or DNA element of interest. If the depositor has... into a backbone, and each is given its own gene/insert section on the plasmid page. Tags are listed according...We Do Quality Control at Addgene Resources on Addgene.org Join the Addgene Community Learn the Benefits...plasmid. Use the Addgene catalog number to easily find template citations for Addgene plasmids you have...

  2. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Horizontal gene transfer (HGT) is the movement of genetic material between organisms. It plays a key ... comic, when a bacterium uptakes a plasmid, the genes on the plasmid can give them different phenotypes...this plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic...used to create both transient cell lines, where a gene of interest is expressed but not integrated into...Conjugation was the first extensively studied method of gene transfer and was discovered in 1946 by Joshua Lederberg...uptake of genetic material from the environment by bacterial cells. In nature, this genetic material often...the donor can transfer genetic material to the recipient bacterium. The genetic material transferred is...

  3. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of interest...) validate successful mutation of your favourite gene, there are several different methods available. ... for complete functional knockout of your target gene. Mismatch nuclease assays are completely “blind”...U., Hauber, I. & Fehse, B. Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer...et al. Digital detection of endonuclease mediated gene disruption in the HIV provirus. Sci. Rep. 6, 20064...validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard.... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  4. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... et al. Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in...Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends on their...Altogether, AAV has many excellent features for human gene therapy and is currently being used in clinical ...in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson, 1982)....

  5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...limits the delivery of larger transgenes, such as base editors which have a gene size of ~5.2 kb. To overcome...Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging...dosage comparable to those currently used in human gene therapy trials. They further tested the base editors...expression levels from different promoters, and inhibited gene expression using CRISPRi. The kit functions in the...that increases temporal and spatial precision of gene expression and reduces toxicity as compared to previous...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...splitting removes any exogenous sequences and regenerates a native peptide bond at the split site. ...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are great ways...the plasmids at Addgene! A new tool for synthetic biology uses CRISPRi to control gene expression. This...nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are ...selectivity and found that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the... ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences. But these...-IgG1-Fc, Addgene #140700) pMAK463 (NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) ...Find these AAVs at Addgene New controls, biosensor, chemogenetic and optogenetic AAV for expression ...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...expression time. 2. Timer functionality with a single gene Although it is possible to make a timer system composed...cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing pancreas by placing...FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation) in mouse...(trafficking to a subcellular location, start of gene expression, development of a cell structure, etc...formation of limbs are correlated with changes in gene expression. In a developing embryo, for example,...long-lived transcription factors control gross changes in gene expression as new body sections are formed. In synthetic...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...

  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...confounding mutations, the risk is high. You are generating a gene therapy product with CRISPR to (hopefully)...not to cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide ...which involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...involves making a large sgRNA library to thousands of genes and targeting and sequencing millions of cells. ...confound an experiment due to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this...Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol Ther. 2021 Mar 3;29(3):937-948. doi... Gao F, Guo Y. Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cpf1 multiplexing method, other multiplex CRISPR gene editing methods relied solely on Cas9. Overall, ...Lastly, an AAV vector was used to multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo...summary of multiplexing efficiency in vivo. Multiplex gene editing with CRISPR-Cpf1 is one of the latest developments...transfection, viral transduction) for simultaneous multi-gene editing with a single crRNA array. Are you interested...        References 1. Zetsche et al. "Multiplex Gene Editing by CRISPR–Cpf1 Asing a Single crRNA Array...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

  10. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer to design...multiplicity of infection (MOI) for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1....with your virus and assaying for expression of a gene carried on the transfer plasmid or quantifying the...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... to contain 1 integrated copy of the qPCR target gene. Developing the proper control line typically requires...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide... that specific feature. Primers targeting the transgene are also beneficial as they ensure that the correct...
Showing: 221 - 230 of 928 results