...PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely useful but is...such as in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...replicates. ddPCR can be used to quantify DNA sequences that are rare, for example, rare alleles or mutations...

... repeating elements anymore. The kit contains sequences for several different fluorescent proteins. Visualizing... of work on wheat rusts and we are collecting sequences that will provide a similar resource for those...they aren’t sure how to convert their own coding sequence to the MoClo system. It’s quite straightforward...

...pH can denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...

...scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental data tables...query screen data without the need to analyze raw sequence data.  This format is inherently flexible and ...

...series. Single DSBs resolved by NHEJ at coding sequences often result in loss-of-function frameshift mutations...analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting to detect. Another...

...targets 18,080 human genes with 64,751 unique guide sequences to enable both negative and positive selection...proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips and tricks Wang offers this...

...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

...Pennsylvania independently searched for this unique sequence of DNA and both identified lymphocyte antigen ...as a receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was ...alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to...

...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

...antibodies will come with access to the antibody sequences, in line with the rest of Addgene’s recombinant...