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Showing: 441 - 460 of 685 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

  3. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...indicator of transcription within cells, where detection of the reporter protein or its enzymatic activity...regions on gene expression can be determined by detection of the reporter in a specific assay, which ideally...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

  9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  11. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  12. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  14. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to...

  16. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...

  17. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ...

  19. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet.   References...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...
Showing: 441 - 460 of 685 results