...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...plasmid encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

...studies in high profile journal articles, many researchers remain unaware that EGFP/GFP is prone to forming... molecules within a volume. In many biological research situations, the relevant volume is that of the...There are some simple solutions available for researchers to deal with all of these problems. The simplest...

...hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre Marillonnet share...and since we are making all the tools for plant research here and validating them, it made the most sense...academic in literature who gave a talk to the women’s research network here. She talked about the advantages ...

...variants, and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many ... & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor...to Our Guest Blogger! Gal Haimovich, PhD, is a research fellow in the lab of Prof. Robert Singer at Albert...

...delivered using this vector. For example, although researchers know that Usher Syndrome (a disease that results...encouraging. Importantly, a significant amount of basic research is being done to further improve the technology...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014...

...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...they’re seeking someone who has, to a t, all the research skills under your belt from your last job. You...managing inventories in the lab, or organizing a research project, one can assume that you’ve had to flex...

...accessibility (Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural...Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses... microscopy: principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038...

...blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...Lavis is currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would...

... by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance...’re turning the tables on the bacteria. Future research will show if this new strategy can help us win... on Addgene.org Find CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design...

...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced... Marcy Patrick...

...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...

...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...

...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...

...Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference, and the...Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc Sze, Victoria McGovern, and Julie Wolf. Apologies...

...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...

...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...

...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...

...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...

...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...