We narrowed to 517 results for: ATO

1. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

2. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

3. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   Published

   Sept. 24, 2024, 1:15 p.m.

   ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

4. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

7. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

9. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

10. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

13. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

15. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

17. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

18. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

19. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...