We narrowed to 512 results for: ATO

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

2. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

3. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

4. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

6. Academic vs. Industry Postdocs

   Type

   Blog Post

   Published

   Nov. 13, 2018, 1:22 p.m.

   ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

7. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

8. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

10. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

12. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

13. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

14. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

15. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...

17. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

18. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...