...Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital...Church and Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD...and explains why he loved the first 2 years of medical school. 7:05 - 10:55 : Alex's focus during graduate...postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to...

...use as a genetic engineering tool is frequently called recombineering - short for homologous recombination-mediated...insert site (Figure 2, top).  These primers are typically ~70 nucleotides long (20 nucleotides that anneal...fragments, such as gene replacements and tags.  The typical frequency of recombinants is 1 positive clone out...efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA substrate ...below).  Controlling expression of Red proteins is critical for minimizing the toxic effects of Gam expression...

...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic...validation process, also known as CRISPR genotyping, is critical to demonstrating causal relationships between ... forms of off-target analysis in order to meet clinical approval. It is key to maintain a consistent set...the detection of KRAS mutations: diagnostic and clinical implications." The Journal of Molecular Diagnostics...

... is necessary for bacterial transformation of chemically and electrocompetent cells is minimal (picogram...nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used during the purification... preparations for this technique must be larger scale, to allow the extraction of enough plasmid to last...addition to being free of any nucleic acid and chemical contaminants, these DNA samples must also be entirely...

...in Plasmids 101, tags can help you determine localization of a protein of interest, purify it, or determine...major caveat - a tag may interfere with protein localization and/or function, so each tagged protein must...proteins found that 20% of tagged proteins did not localize similarly to their untagged counterparts. These...fluorescent-protein tagging show high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013...

...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands,” ...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their..., the brain is protected from stress-induced biochemical changes that could otherwise lead to depression...

...Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining the problem... training too many scientists and some propose radical changes to the way we view the graduate school ...University founded a successful consulting company called The Balsa Group. University of Pennsylvania has...

...paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford University...as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution...I have worked many hours in a dark room with a confocal microscope, yet I was once again fascinated by...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...

...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop...FierceBiotech to “learn the business” Attend events with local networking and trade organizations Another excellent... be found on the UK Career Site for Researchers called Vitae. Vitae recently published a career development...

...pride in facilitating the exchange of not just the physical samples, but also the legal documentation that... Addgene has adopted the terms of the Uniform Biological Material Transfer Agreement (UBMTA) for its material... may not use the material in human subjects or clinical trials Acknowledgement in recipient’s future...have opted into our eMTA system can even sign electronically or choose to auto-approve MTAs. Whatever your...

.... All of this requires genes to be regulated dynamically.” To understand how that works in memory formation...they could package LITEs in viral vectors and genetically target them to specific cell populations. They... the genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids...with LITE? Reference: Silvana Konermann et al. "Optical control of mammalian endogenous transcription and...

...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211...launched AAV Packaged on Request, a service that dramatically expands and simplifies your viral vector options..., K., Alshafie, W., Baker, M. R., Ball, A. R., Callahan, D., Cooper, J. A., Crosby, K., Harvey, K. J.,...

... therapies,” said Kris Wood of Duke University Medical Center. “While we do not yet have a comprehensive...resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully, the researchers...generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and it took them... he said. “It could [be used to screen for] morphological features, migration, or survival in different...

...while - a tool to expand perception, which is ironically what science is about. For me it is essential...living organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...the lab with parties in the lab’s common areas, local pubs, and homes. The Church lab also toasts a newly... Improving your work-life balance Balancing the Scale: NSF's Career Life-Balance Initiative Can Scientists...

...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...upcoming grant deadlines, and sync everything to your calendar. Ditch the paper To Do List and move to one that...reminders. These programs can be synced to your online calendar and can be accessed from your computer or smartphone... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...

...nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long ...by direct transformation. A given insert will typically contain both a protein modification sequence and... not only track the abundance, movement, and localization of individual proteins, but also investigate... green, and red FPs that are codon optimized specifically for expression in yeast. These tagging vectors...

...expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and...feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors cannot be...nLightR, both generating a measurable signal after electrical stimulation with a single pulse, while nLightR...Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific...

...imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging...not recommended reagents) along with information critical for the performance of a reagent, e.g., antigen... (2022). IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse...Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist...