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We narrowed to 694 results for: EED

Showing: 441 - 460 of 694 results

  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...

  3. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...CHRM4 gene. When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing...interrogating the VGS data for small predetermined seed sequences unique to the capsid plasmid. Our recombination...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from... your colonies are well separated. For this, you need to have some idea of the rate of growth of your ...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... pose the greatest threat, cancerous cells still need to successfully infiltrate the circulatory or lymph...include several other important pathways. We invite feedback and contributions from all members of the research...

  6. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...

  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...is the point?  Antibodies are large proteins that need a little help crossing cell membranes as they are...the inside of the cell. However, assuming you do need to permeabilize, you have a few options for what...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  13. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ...

  14. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...

  15. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...between the size of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the...protein. In some cases this property eliminates the need for removing excess dye from the sample, which is...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide...

  18. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...immediately transformed and stored. Some samples need to be transformed with a micropulser using an electric...samples require a reduced antibiotic concentration or need to be transformed into different cells than were...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance...
Showing: 441 - 460 of 694 results