We narrowed to 700 results for: EED

... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...

...tag reduces the number of CAPTURE components that need to be delivered to cells from three to two. Lentiviral... remaining CAPTURE 2.0 components eliminates the need to create stable cell lines and allows CAPTURE 2.0...

... reagents in three major steps - all without the need to find a llama farm! To find an optimally binding... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

...system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

...to knock in corrective genes. For this you would need to provide a DNA template to repair the double strand... homology directed repair and you would probably need to use a two viral vectors system: one vector for...

...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

... pose the greatest threat, cancerous cells still need to successfully infiltrate the circulatory or lymph...include several other important pathways. We invite feedback and contributions from all members of the research...

...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...

...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...

...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

...is the point?  Antibodies are large proteins that need a little help crossing cell membranes as they are...the inside of the cell. However, assuming you do need to permeabilize, you have a few options for what...

...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ...

...between the size of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the...protein. In some cases this property eliminates the need for removing excess dye from the sample, which is...

...world, coordinates alone aren’t very useful. We’ll need something like Google Maps to help us identify and...NANOG enhancers from step 1 with genome math, we’ll need a bit of shorthand from the field of epigenomics...

...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide...