Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 856 results for: ELL

Showing: 441 - 460 of 856 results

  1. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...with a great story to tell? Let us know by emailing us at [email protected] or tell us on Twitter and Facebook...amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and explaining...better day at the office but for stronger science as well. This blog is our way of continuing the conversation...whether you’re a graduate student, post-doctoral fellow, lab manager, research technician, or Principal...conversation! Do you have a question about culturing iPS cells? Confused by which system to use for genome editing...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...benefit, replicates are built into the technology. One well (containing thousands of droplets) can be sufficient...fluorescence amplitude measured for each droplet in each well (Figure 2). A clean ddPCR should have a clear separation...negative (gray) droplets. The no template control wells should have very few positive droplets. In the image... copy per microliter in the no template control wells. You’ll also notice a reduction in the number of...cross contamination into a no template control (NTC) well is common. Here are some steps you can take to achieve...applications for the technology.  Droplet digital PCR is well suited for the detection of low copy numbers. For...used in the detection of rare sequences and single cell analysis. It is also being used in the detection...

  3. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... Orientation-Specific Tells you the orientation of the insert Tells you if the insert is present...product. Additionally, this type of primer only tells you if your specific insert is present but not if...without the insert. This type of primer pair can tell you if the insert is the correct size and whether...Insert-Specific Simple "yes or no" test results Doesn't tell you the orientation of your insert or if the insert...band (-) result as insert-specific primers but also tell you whether the insert has the correct directionality...clones you’ll need to send for sequencing, but won’t tell you if your products have any mutations.  There ...

  4. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...processing, the window shows a yellow checked circle. Typically, a yellow check mark indicates that a particular...score (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This...Following delivery of CRISPR components into target cells, genomic DNA from both edited and unedited (control...in front of that sample. You can hover over the yellow or red circles to gather details on the issues ... score. KO Score - Represents the proportion of cells that have either a frameshift or 21+ bp indel. This...vertical dotted line represents the cut site, and yellow + symbol on the far left marks the wild type sequence...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...homology-directed repair (HDR) pathway which occurs in cells at low frequencies and relies on the efficient delivery...Later the resultant U-G pair is either repaired by cellular mismatch repair machinery making an original C-G...editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful evolution of...’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic... is converted to a G-C pair (Figure 1). As the cellular inosine excision repair is not as active as uracil...protospacer, ABE6.3 or ABE7.9 are better suited (Gaudelli et al., 2017). Our vectors have two Bsa1 sites...353.6305 (2016): aaf8729. PubMed PMID: 27492474. 3. Gaudelli, Nicole M., et al. "Programmable base editing ...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...a review article on histone modifers in B and T cells with my adviser when I was a graduate student. Naturally...aspect of the field you plan to review. If it’s a well-researched field, you may need to get specific to...these themes, and also for articles from the active/well-known researchers in the field. I made sure to find... find information from genome-wide studies, as well as results from smaller and more specific studies....studies. I also did not limit myself to the well-cited or popular papers, but looked for papers from a wide ... change the sentences, make it flow, and start telling the story. Like any editing process, you will need...acetyltransferases and histone deacetylases in B-and T-cell development, physiology and malignancy." Genes &...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... attack the cell wall of bacterial cells; however, Mycoplasma species do not possess cell walls. As a ...bacteriophage (viruses that only infect bacterial cells), or a bacterium picking up resistance genes from...determining drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical...conserved, non-negotiable process within the bacterial cell, antibiotic resistance is very likely to develop...globally: final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information...

  8. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...lipopolysaccharide of the cell wall of Gram negative bacteria like E. coli that affects tissue culture cell line viability...introduces transformation grade plasmids into a bacterial cell. Transformation grade DNA  Transformation is ...transformation of chemically and electrocompetent cells is minimal (picogram range). It can be extracted...Figure 3: Need to introduce plasmids into mammalian cells? Use transfection grade DNA. Transfection is ...process of inserting an external plasmid in mammalian cells and it will likely require micrograms of plasmid...

  9. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...both Cas9 and your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer...knock-out thousands of genes in mixed population of cells as part of pooled library screens (1,2). The human...pooled libraries targeting both mouse and human cells are avaliable for distribution. To learn more about... includes work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase...Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166 (2014): 84-87. PubMed PMID: 24336571...Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral...

  10. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...It’s well known that exercise can help fight stress and depression, and now researchers have reported... how it works. The findings in a recent issue of Cell demonstrate how PGC-1a1, a transcriptional coactivator... press release. “We actually found the opposite: well-trained muscle produces an enzyme that purges the... in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty more...including psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about...JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016/j.cell.2014.07.051. PMID: 25259918 Muscle over mind. Moon...

  11. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Addgene Outreach Scientist and fellow blogger Caroline LaManna put it well: "Wow, custom mutations in monkeys...RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) Programmable...correct disease mutations, both in mice and in human cells. But that important advance was quickly followed...  David S. Weiss of Emory University summed it up well in that New York Times feature when he said, “All...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...activity which cleaves the supplied fluorescently labelled ssDNA substrates. Upon cleavage, the fluorescence...complementarity between the gRNA and a given fluorescently labelled substrate, gets activated and performs non-specific...strips to detect the cleavage of fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence... with streptavidin coated beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no ...Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems in the Sashital...2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst JA, Granados ...Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...rats have some key differences in physiology, intelligence, and human correspondence that give them an ...routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016). For these reasons rats were ...transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed several vectors for...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...https://doi.org/10.7554/elife.50651 Vieira, W. A., Wells, K. M., & McCusker, C. D. (2019). Advancements to...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...translation of many stop codons and detrimental effects on cell growth. The C321.ΔA strain was genomically recoded...in stability of the p-Ser incorporated protein as well as context-specific effects of UAG suppression, ... Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison...is particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion..."The origins of protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID: 11988757...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ... by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to a team effort.... 5. Five Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms...

  16. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...Or you don’t know how well your assay will scale. Or you don’t have enough cells to execute genome-wide...spreadsheet of random numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library...genome is limited by definition, the kinome is a well-annotated collection of genes that can provide a...lentiGuide expressing only an gRNA, for use with cells that already have Cas9). Each library consists of...be useful when screening for phenotypes, such as cell-non-autonomous effects, that do not lend themselves...

  17. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...How do you celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams...-off via a gift, card, and trip to the pub. Dr. Ellis adds “we probably don’t celebrate enough... I’ll... pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with dinner and champagne.... What does your lab do for fun as a group? The Ellis Lab self-organizes cinema nights, game nights, and...retreat. How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and...from you! Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...desired mutation in mouse ES cells, select for the mutation and inject the ES cells into a mouse embryo to ...Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell.2013.08.022 PubMed: PMID: 23992847...then electroporate it into mouse embryonic stem cells. After selecting for clones with the recombined ... your chimeric mice did not contain edited germ cells, your experiment would be unsuccessful. CRISPR made... targeting the same or complementary strands, as well as guides facing towards or away from each other...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...practice (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however...projects, this can cause conflicts and favoritism, as well as affect morale and cause gossip). These kinds ...that, when those contracts fall through or are cancelled, then redundancies can be made within the company...  Working abroad: Research in Australia I then travelled abroad to work at the University of Queensland...customer service (recruitment agencies often don’t tell you what company a job is with until just before...towards the scientific aspects of the company as well and use my other skills and interests. I was so ...flexibility of a ‘start-up’. I was surprised how well I fit in with the company and that my ideas and ...

  20. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...professional and personal development, is essential to excelling in the modern world. Talent knows no boundaries...me in my next steps. For example, how to get a fellowship to travel abroad, how to write cover letters,...mentees identified career opportunities in science as well as the developmental needs and resources needed ...the program included improvements in writing fellowships, giving oral presentations, and preparing for...profesional networks, and nominations for government fellowships. The closing and formal evaluation of the pilot...Spanish government’s support for the IMP program as well as for the Spanish scientific community overseas...particularly interested in breast cancer and metastasis as well as teaching and mentoring activities. Follow her...
Showing: 441 - 460 of 856 results