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TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...
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Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m...., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m.... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....about what reviewers in your area are looking for. Remember though, you need to leave them time to read it... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m.... money on a potentially faulty product. Please remember that the person at the other end of that email... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m.... environments. Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m.... protocols attached. Final step: validation Remember, there is no “one-size-fits-all” protocol! Each... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....But we hope this guide can help you get started. Remember, you can always change your compensation values... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....When considering what method you’d like to use, remember that the final decision will likely come down ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... We invite feedback and contributions from all members of the research community as we continue to to ... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....positive. I went back into lab the next day and remembered why I still love science. Keep building on your... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...