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We narrowed to 685 results for: Eng

Showing: 441 - 460 of 685 results

  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

  3. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...determinants of prime editing efficiency such as the length of the RTT overhang, the GC content of the primer... pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some...

  5. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...models in species that were previously difficult to engineer, like rats. If you have tools for creating knockout...

  6. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  7. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...

  11. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...viruses, with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of...2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...scientists of all types to stay up to date and engaged with new advances in published science. They also...

  16. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could also...://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao...

  19. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    .... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...
Showing: 441 - 460 of 685 results