We narrowed to 738 results for: HA
-
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....relatively confident that the gene from the test species has a similar function to the gene in the reference species...left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell biology...
-
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....your data to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your... doors Getting involved in science communication has been the most rewarding step I have taken in my career... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....particles with polybrene, a cationic polymer. Polybrene has long been used to increase retroviral transduction...of mouse zygotes and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....training. Addgene executive director Joanne Kamens has written a 5-part blog on the topic: http://blog....people mostly online and on monthly phone calls. This has its challenges as meeting in person is often much... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....(deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular cloning... -
Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart
TypeBlog PostPublishedMarch 9, 2018, 2 p.m....Professor Goedhart is very active on Twitter and has helped us update many of our educational resources... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m....pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog PostPublishedJune 13, 2019, 12:19 p.m....Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to ... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....we highly recommend checking out the video). Tom has made many more Science rap videos to teach students... -
Find and Share AAV Data with Addgene's New AAV Data Hub
TypeBlog PostPublishedOct. 8, 2019, 12:33 p.m....injection before performing the experiment. Every entry has an accompanying image so that you can see for yourself... -
Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m.... crucial environmental research programs, Canada has been here before. These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that...emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation at another...2023). The field of fluorescent ligands and dyes has witnessed remarkable advancements, driven by the ... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m.... inhibit these viruses. But llama immunization has its drawbacks: it requires a camelid animal facility...selection step using ribosome display because it has the advantage of starting out with a larger initial... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....toolbox. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA, Solá-Esteves NM, Barrera LA, Born DA, Edwards A,...