...Professor Goedhart is very active on Twitter and has helped us update many of our educational resources...

...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

...we highly recommend checking out the video). Tom has made many more Science rap videos to teach students...

...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to ...

...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along...

...injection before performing the experiment. Every entry has an accompanying image so that you can see for yourself...

...used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters...

... crucial environmental research programs, Canada has been here before.  These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many...

...inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that...emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation at another...2023). The field of fluorescent ligands and dyes has witnessed remarkable advancements, driven by the ...

...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...

... of stem cell potency: Totipotency: When a cell has the ability to produce all of the differentiated ... are totipotent [2-4]. Pluripotency: When a cell has the ability to differentiate into cells of any of...

... inhibit these viruses.  But llama immunization has its drawbacks: it requires a camelid animal facility...selection step using ribosome display because it has the advantage of starting out with a larger initial...

...toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA, Solá-Esteves NM, Barrera LA, Born DA, Edwards A,...

...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

...useful in an experiment, and want to see if someone has already created and deposited it with Addgene. Combining...resistance gene(s) used for selection in bacteria. Has Viral Service?: Allows you to filter results to only...

... to edit genes in several biological systems. It has been used for instance in inherited disorders such... is often used, the CRISPR approach based on HDR has the advantage of being a potentially universal treatment...