We narrowed to 457 results for: PAC

1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ...the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test...

2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...into newly synthesized DNA. This labeled ssDNA is packaged into phage and then extracted. Besides taking ...

3. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...more model organisms that have left a historical impact on the scientific community. (And, check out this...

4. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...

5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...development of a new technique. In a structure as tightly packed and interwoven as the brain, robust single-cell...

6. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   Published

   Oct. 2, 2018, 12:56 p.m.

   ... this. The training you get now will have a big impact on everything you do in the future. Questions to...

7. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

8. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...I really loved the fact that my work had a real impact  people’s lives and would improve their treatment...

9. Deep Dive: Statistical Tests (Comparisons)

   Type

   Blog Post

   Published

   March 7, 2023, 2:15 p.m.

   ...independent variables. For example, you could test the impact of temperature, humidity, sunlight exposure time...

10. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...specificity. However, there are countless variables that impact an antibody’s affinity and specificity for a given...

11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...the most prevalent method due to ease of use and capacity to generate a long-term RNAi phenotype. Long hairpin...

12. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...stability. Additionally, the choice of vector can impact the expression level of an antibody (Ayyar et al...

13. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...imaging, the light microscope was limited in its capacity to resolve closely lying structures. This is because...

14. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...GC%, reverse compliment sequences, and more. Output_Compact: A text file that describes which genes were...

15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...

16. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...with a unique Deposit ID. Addgene will send you a package containing instructions on how to prepare your ...

17. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...optimal scaffold can depend on the length of the spacer region between the target sequences. These updates...