We narrowed to 456 results for: PAN

1. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ...

2. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ...publication at Addgene. Riabinina, et al. “Improved and expanded Q-system reagents for genetic manipulations.” ...

3. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute...

4. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ... digestion varies for different enzymes. Many companies now sell fast digest enzymes that can digest large...

5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ...assay kits are available from different biotech companies which are enzyme-based mycoplasma detection kits...

6. Antibodies 101: Antibody Engineering and Directed Evolution

   Type

   Blog Post

   Published

   Aug. 5, 2025, 1:15 p.m.

   ...diversity of antibody reagents is continuing to expand, thanks to the efforts of antibody engineers. Many...

7. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ...transposon in vertebrates. It was discovered in Japanese medaka fish because its insertion into the fish...

8. Important Considerations When Using AAVs

   Type

   Blog Post

   Published

   June 13, 2017, 2:30 p.m.

   ...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 ...

9. Typing CRISPR Systems

   Type

   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ..., Y., & Fu, Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing...

10. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...simply cease to exist. But then November hit and panic ensued as “alternative facts” became a part of our...

11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...their degradation within labelled cells limits the timespan available for tracking certain cell types. Another...

12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...stem loops bind along the outside of the protein, spanning both lobes. Stem loop 1, nestled between the REC...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins, Mark A. Kay,...

14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ....  Cytidine deaminase for Pseudomonas species Expanding this tool to other organisms, the Ji lab brought...

15. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...response over a broad dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic...

16. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...synaptic step, thus preventing further widespread expansion of RABV. This improvement resulted in a clearer...