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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 860 results for: REN;

Showing: 441 - 460 of 860 results

  1. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...He mixed together 10 E. coli strains producing different pigments, and tasked the students to separate ...over 20 years. Previously, the lab used three different species for the challenge, but one species always...Addgene is have the students transform them into different strains of E. coli,” says Westenberg. During the...transformation experiment, he plans to show how different genetic backgrounds of the host strains affect...

  2. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...“NCBI Site Preferences” box. Once on the Preferences page, navigate to “PubMed Preferences” and click ... that you know how to navigate to the PubMed Preferences and Filters pages, be sure to check out the other...

  3. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...requested plasmid, the number of requested plasmids, or trends of requests over time. Visualizing the data So,...is a great step up, but I needed a way to show different aspects of the data simultaneously. That’s why...dashboards are (i) easy access to important numbers and trends, (ii) user interaction with the data, and (iii)...new data. These features are incorporated in the current dashboard. The dashboard is coded in R Markdown...

  4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...allele is selectable, it can be transferred to a different genetic background via P1 transduction. Plasmid...could be used to move the modification into a different background, if needed. This approach is best suited...our guest blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory...science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced levels of...Engineering in Bacteria Using Homologous Recombination. Current Protocols in Molecular Biology. 106:V:1.16:1.16.1...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...ultrasound dyes because these dyes “collapse” at different ultrasound frequencies. Read more about ‘Using...created that produces loads of inspiration for the current and the next generation of microbiologists. If...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter...

  6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...numerous cell lines can be generated, each with a different tagged protein of interest, and a universal all-purpose...cells (Bressan et al. 2017; Liang et al. 2017). Current methods of epitope tagging, however, suffer from... achieve 5-30% tag knock-in efficiency across different stem cell types. This has facilitated easy generation...format and tested parallel tag knock-in for 96 different genes in a single go (Figure 3). We were able ...seen daydreaming about sunny days in the UK.   References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso...

  7. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...mentor. Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors... of a good mentor: Yoda image courtesy of jorenl/Vecteezy.com Helps set concrete goals and ...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by...

  8. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...the Addgene repository and their choice of five different serotypes.   Figure 1: The process of... this service.  At launch, we will offer five different serotypes for packaging: AAV1, AAV2, AAV5, AAV8...that this ordering process will look slightly different from our standard process. We’ll walk you through... Preps will be available for request in three different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5...

  9. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...plasmids for expressing different types of rhodopsins and sensors as well as references and links to other ...using both its Addgene ID number as well as the reference for its original publication. To help facilitate... citation that you can put straight into your reference section. Why do this? Not only does citing plasmids...

  10. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...eyes to the many different jobs associated with science, especially if the conference is a big multidisciplinary...curious and interdisciplinary.   3. “There are many different ways to succeed – as loner or gregarious;  dictator...leaving academia should present their research at conferences to obtain a “crash-course in all the real life...

  11. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...online on Addgene's CRISPR Library Reference page.    References: Genetic Screens in Human Cells Using...as an answer to that question. Additionally, a concurrent Nature Biotechnology article describes the development...GeCKO library, each library has been enriched for different gene targets. These pooled libraries include enrichment...

  12. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...of gene editing. Bon voyage!   References and Resources References Gelsinger, D. R., Vo, P. L. H., Klompe...downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular bacterial ...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...

  13. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...

  14. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...tools for your next experiment! References and Resources References Wood, V., et al., Hidden in plain...this many genes are truly dispensable or are the current identification methods missing something? Here ...an endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...

  15. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...but in time I realized its value. My master’s strengthened my critical thinking and problem solving abilities...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...generalist? Working on a variety of projects and in different fields of research is one of the best parts of...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...similar purposes, both techniques offer researchers different advantages and limitations.  The four main factors...study. Figure 1: A schematic showing the differences in chemogenetics and optogenetics in a mouse ...manipulated or turned off. In chemogenetics, while different concentrations of ligands can be injected, this... article and sharing their expertise with us. References Urban DJ, Roth BL (2015) DREADDs (Designer Receptors...

  17. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...buffer, or some other variations. Does it make a difference? Short answer: yes. This last step will influence...preparation can be eluted in water or in your buffer of preference, and it will be stable for years. Plasmid DNA...choose the best way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage... Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids...

  18. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...Open Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell lines...community, but also allows cross-interrogation of different datasets that might otherwise be hard to identify...breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot updated 2024...to analyze raw sequence data.  This format is inherently flexible and allows the broadest possible scope...

  19. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...compare and analyze any differences between our sequence results and the reference sequences. Using Snapgene...features To align NGS sequencing results with reference sequences To design experiments for further plasmid...Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...

  20. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Side-by-side comparison of the same experiment from different cell lines/model systems ReFigure allows you ...access life sciences data. You can use ReFigure to coherently save analyses with the relevant figures from ...submitting your data to a repository (Figshare is currently supported by ReFigure beta) you can include it... in a few minutes, visit our YouTube Channel. Currently we can only connect figures from PubMed Central...
Showing: 441 - 460 of 860 results