We narrowed to 453 results for: Spr

1. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...

3. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...

4. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...

5. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific...

6. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...

7. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab doing experiments...

8. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

9. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources...

10. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...; once that seed of distrust is planted, it can spread like kudzu. Think of a soccer player who gets a...

11. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...Handbook of biological confocal microscopy, 3rd edn. Springer (2006). 7. Yang, Weijian, and Rafael Yuste. In...

12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...

13. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO...