We narrowed to 543 results for: TIL

1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment The Won...

2. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans." ...

3. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...envelope plasmids here. Lentiviral vectors are very versatile tools as they are able to transduce dividing and...

5. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

6. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...role in helping to monitor and slow this spread until vaccine programs can be put in place to provide ...

7. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes carried on transfer...

8. Gaining Leadership Skills Volunteering at a Professional Organization

   Type

   Blog Post

   Published

   Aug. 6, 2015, 2:30 p.m.

   ...invaluable experience and even after 5 years, was still improving my skills. My involvement with the NPA...

9. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ...capabilities. So don’t throw old equipment away until you ask your local makerspace if they want to hack...

10. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...because of the second passage and because you wait until the majority of cells have died to collect your ...

11. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...

13. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...canceled, taking advantage of virtual meetings can still be a great opportunity to introduce yourself and...

14. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...saving both time and effort. Scientists can also utilize multiplex qPCR to quantify the concentrations of...

15. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...myo-inositol oxygenase), providing enzyme protection while still allowing substrates and products to diffuse freely...

16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...along for the ride throughout all of these steps, until its connection with DNA is broken during cross-link...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ... References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning System for Characterization...

18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

19. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...DNA constructs designed for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust...

20. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...that they were having a positive experience, I was still incredibly nervous about leaving academia. A lot...