We narrowed to 546 results for: TIL;

1. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...cycle once inside the host. While the virus can still enter (and thus infect) cells, this safety feature...

2. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...GFP, our most-requested GFP(1–10) and GFP(11) Versatile protein tagging in cells with split fluorescent...

4. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different...

5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering...

6. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a recombinant...

7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...targeting the same cell class in all three species are still greater than random chance. The chances of a promoter...

8. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...role in helping to monitor and slow this spread until vaccine programs can be put in place to provide ...

9. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes carried on transfer...

10. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...invaluable experience and even after 5 years, was still improving my skills. My involvement with the NPA...

11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...capabilities. So don’t throw old equipment away until you ask your local makerspace if they want to hack...

12. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...because of the second passage and because you wait until the majority of cells have died to collect your ...

13. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and...

14. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...DNA constructs designed for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust...

15. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    .... If you don’t have access to a list of posters until the day-of, take a moment to step aside to read ...

16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...along for the ride throughout all of these steps, until its connection with DNA is broken during cross-link...

17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ... “spectral flow” cytometry, on the other hand, utilizes the full spectrum of light to distinguish one ...

18. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...canceled, taking advantage of virtual meetings can still be a great opportunity to introduce yourself and...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, ...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...a minute, when it is only warm but not hot- and still liquid- it is rapidly poured onto the middle of ...