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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 693 results for: Tec

Showing: 441 - 460 of 693 results

  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

  8. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

  12. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

  15. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  18. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to...

  20. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...
Showing: 441 - 460 of 693 results