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We narrowed to 749 results for: Tro;

Showing: 441 - 460 of 749 results

  1. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...evaluated what lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas ...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest for about...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods for repairing...NHEJ is error-prone, such a system is likely to introduce additional nucleotides flanking the insertion....the DNA ends anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition...

  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...comment, annotate, and edit protocols. After my introduction to protocols.io, I decided to research what ...orders, tracking students and research projects, electronic lab notebooks and protocol management. Though...More Reading: How to manage scientific data: Electronic lab notebooks compared with Google Docs and wikis...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ...

  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...impossible for a career scientist such as Zimmer to introduce any one of these topics without some editorializing...fellow scientists--but that doesn’t make his introduction to these topics any less effective. Who is this...non-scientists in your life, the book may be the introduction they need to grasp the challenges you face in...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...preferred platform before you start can save a lot of trouble in the long run.  Selecting the method Once you... Requires regular content production. Internet "trolls"   More resources on the Addgene blog:  Summer...  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs. This library was designed to improve on-target...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...

  10. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...provided a major toehold into understanding how genes control biology (a fact celebrated by the Nobel prize committee...transgenics Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety...into adults and explain how signaling pathways control this process. Additional zebrafish applications...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...of symptoms in animal models, Rett syndrome is a strong translational candidate for gene replacement therapy...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar...neurological disease (Sinnamon et al., 2020). They introduced a mouse MECP2 guide RNA and a hyperactive version...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...advantages. An introduction to Gateway technology The Gateway cloning method, developed by Invitrogen, is an ...an in vitro version of the integration and excision recombination reactions that take place when lambda...

  13. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...laboratories and associated publications, but our quality control (QC) standards have evolved over the years to keep... ability to re-use or borrow existing code and control how the various pieces were connected, as this ... for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...direction (which is not easy to see in colony PCR electrophoresis), is free of undesired mutations, and that ...

  16. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... for saturation mutagenesis, and they provide a strong proof of concept for further development. ...excision repair pathway. The team behind AYBEv3 introduced the first deaminase-free base editor when they... called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited selectivity...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs... an edited strain carrying Cas9 to allow the introduction of additional sgRNAs. Image reused from Neo ...

  18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...glia-specific intermediate filament expressed in astrocytes throughout the brain, making it a popular target...

  20. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...have Kd around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein...
Showing: 441 - 460 of 749 results