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We narrowed to 919 results for: addgene

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Showing: 441 - 460 of 919 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...line), armed only with viruses obtained through Addgene’s viral service and the methods sections of scientific...of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview...repeatability and bad news for a screen. Because Addgene viruses are provided at a specific titer, you can...Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962...PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide Screening Using CRISPR/Cas9 Deliver...Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell Lines with Lentivirus...

  3. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a... the Cpf1 multiplexing plasmids available from Addgene. If you have any questions or thoughts about Cpf1...Central PMCID: 4638220. Additional Resources on the Addgene Blog Cpf1: A New Tool for CRISPR Genome Editing...in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing Resources Find...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...PubMed PMID: 19185711. Additional Resources on the Addgene Blog Beware of Fluorescent Protein Oligomerization...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes...bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More about Homology Directed Repair... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

  6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  7. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...biology and society.     Additional resources on the Addgene blog Check out our series on careers in science...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...are many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates... Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2...PMCID: PMC4830361. Additional Resources on the Addgene blog Viral vector topic page Beginners guide to...guide to all things lentivirus Resources on Addgene.org Gamma-retrovirus Guide Lentivirus Guide ...

  9. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...TRIM-Away reagents and reporters are available from Addgene - we hope they will TRIM down the difficulty of...Endosomal pH Sensors podcast segment The first Addgene plasmid deposit from Professor Stefan Hell, who...Richardson, recently contributed a relevant post to the Addgene blog. Find the SRpHi Biosensors here. Richardson... Various Addgenies...

  10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check out Addgene’s job openings...but then relax.  Additional resources on the Addgene blog: Tips for writing a good cover letter 6 Steps...

  12. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...References and Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...

  13. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  16. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ...

  18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...Please check out Addgene’s Choosing a Molecular Cloning Technique page and the Addgene Blog for a variety...PubMed Central PMCID: PMC209908 Resources on the Addgene Blog Plasmids 101: Common Lab E. coli Strains ...all of our Plasmid Cloning Posts Resources on Addgene.org Choose a Molecular Cloning Technique Find Plasmid...

  19. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...

  20. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...ability to use rats as disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus...disease alleles during and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes...popular model organisms in the lab, check out Addgene’s plasmid collections to help start your experiments...E-coli-as-a-Model-Organism.aspx. Additional resources on the Addgene blog: Read more about model organisms in the first... in our CRISPR 101 blog series Resources on Addgene.org: Browse our plasmid collection page Find more... Various Addgenies...
Showing: 441 - 460 of 919 results