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We narrowed to 906 results for: addgene

Showing: 441 - 460 of 906 results

  1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...TRIM-Away reagents and reporters are available from Addgene - we hope they will TRIM down the difficulty of...Endosomal pH Sensors podcast segment The first Addgene plasmid deposit from Professor Stefan Hell, who...Richardson, recently contributed a relevant post to the Addgene blog. Find the SRpHi Biosensors here. Richardson... Various Addgenies...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...References and Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...

  4. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

  5. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a... the Cpf1 multiplexing plasmids available from Addgene. If you have any questions or thoughts about Cpf1...Central PMCID: 4638220. Additional Resources on the Addgene Blog Cpf1: A New Tool for CRISPR Genome Editing...in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing Resources Find...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...

  8. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ...

  9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...PubMed PMID: 19185711. Additional Resources on the Addgene Blog Beware of Fluorescent Protein Oligomerization...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes...bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More about Homology Directed Repair... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

  11. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper published...Research @FORsymp.   Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...Please check out Addgene’s Choosing a Molecular Cloning Technique page and the Addgene Blog for a variety...PubMed Central PMCID: PMC209908 Resources on the Addgene Blog Plasmids 101: Common Lab E. coli Strains ...all of our Plasmid Cloning Posts Resources on Addgene.org Choose a Molecular Cloning Technique Find Plasmid...

  13. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...sequence. (A) Plasmid sequence from pLVUHshp53 (Addgene plasmid #11653). Full shRNA sequence is highlighted...boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence is highlighted...button_text" is_json="true" %}{% raw %}"Browse Addgene's shRNA plasmid collection"{% endraw %}{% end_module_attribute...s12551-021-00902-w Additional resources on the Addgene blog RNA Interference in Plant Biology: New Tools...https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org/collections... Plasmids 101 Posts Additional resources on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...vectors (for a more detailed explanation, check out Addgene's Lentiviral Guide). To produce lentiviral vectors...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation... how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...Central PMCID: PMC9377332 More resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle...every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References and resources... to All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...ability to use rats as disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus...disease alleles during and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes...popular model organisms in the lab, check out Addgene’s plasmid collections to help start your experiments...E-coli-as-a-Model-Organism.aspx. Additional resources on the Addgene blog: Read more about model organisms in the first... in our CRISPR 101 blog series Resources on Addgene.org: Browse our plasmid collection page Find more... Various Addgenies...

  17. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    Published
    July 26, 2018, 12:46 p.m.
    ...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

  18. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...PubMed Central PMCID: PMC3969860. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages Browse Our...

  19. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

  20. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...
Showing: 441 - 460 of 906 results