We narrowed to 526 results for: arl
-
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target of intense...
-
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....plasmid has been requested by labs around the globe nearly 400 times. Researchers have been excitedly testing... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP, cyan... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....Indiana University School of Medicine. She is particularly interested in science communication and fundamental... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m.... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....www.protocol-online.org/ Protocol Online is one of the early protocol sharing websites. It was created in 1999... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....adventure, just remember that even though these early steps don’t even involve antibodies, they are still... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m..... Most notably, China was affected by COVID-19 earlier than the rest of the world, from mid-January to... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs.... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...