We narrowed to 512 results for: ato

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

2. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

4. Academic vs. Industry Postdocs

   Type

   Blog Post

   Published

   Nov. 13, 2018, 1:22 p.m.

   ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

5. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

6. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

8. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   Published

   Aug. 29, 2019, 12:50 p.m.

   ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

10. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

12. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

13. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

14. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...

15. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...

17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

18. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...