We narrowed to 462 results for: bli
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TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...
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Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m.... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....discovery will need, and how long it will take to publish based on these findings. Most often we suggest ... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....r-m+) MG1655 Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....the payload sequence into the genome. The team established that they could reprogram the genomic target ... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....The following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m.... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m.... generations of lentiviral vectors have been established so far, with safety increasing in each generation... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m.... of the double-floxed opsin construct, thereby enabling its expression under a strong promoter only in... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m.... or boards, support ballot initiatives, deliver public comments, meet with local representatives, or even... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....unless you share the results with others. If you publish data generated using your antibody, it’s best scientific... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m.... of comparisons. The Bonferroni does this by establishing a significance threshold at a p value less than... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....CRISPR use grows across the life sciences and to establish standardized validation techniques for academia... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m.... use in mammalian systems, with the landmark publication in 2013. Since that point, it has become the ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....495/517 nm. FITC binds to an amine on a protein, enabling the labeling of protein substrates/peptides and... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....the many primary research papers that have been published at this point. In brief, some standard opsins... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....around the same time. (The papers were actually published in the same journal on the same day.) This team...