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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 807 results for: cha;

Showing: 441 - 460 of 807 results

  1. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...Plasmids Isolated from Lactic Acid Bacteria:Their Characteristics and Potential Applications in Biotechnology...

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...by the general public (either through taxes or charitable donations) so there is an increasing realization...funding or not; applications for funding from charities, commercial organizations, or universities may...-doc at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, ...interference (RNAi) is an RNA-mediated gene silencing mechanism. Scientists can express siRNAs to direct the cellular...that can reveal how genetic alterations lead to changes in phenotype. The plasmid comes with a 1.9 kb stuffer...

  4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...a DNA sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore...retroviral transduction efficiency by neutralizing the charge repulsion between virions and the cell surface,...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...NGS in 2017 for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

  6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...longer Cas remains active in a cell, the greater chances there are for off-target edits to occur. Although...effects Prolonged Cas activity can increase the chance of off-target editing particularly after the majority...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

  8. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...publications. Bonus: papers have to be summarized in 280 characters! Using Twitter to find papers  You’ll need to...a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional...

  9. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...will be greatly reduced, allowing for clearer characterization of the location, morphology, and circuit mapping...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... protein aggregation, you may want to consider changing your incubation temperature. Some proteins aggregate...kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane size is probably the easiest troubleshooting...alcohol. The increased SDS will increase the negative charge on the protein, allowing it to move more readily...Posttranslational modifications Check literature Highly charged amino acids Run control protein (if available...

  11. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...

  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing PAM... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

  14. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...individuals in your team you will have a much better chance of creating a high-performing team - which is every.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... be translated at the ribosome into polypeptide chains that make up the primary structure of proteins.... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... about his/her background. This will give you a chance to determine how to tailor your presentation so...sought out your poster, but will also give you a chance to slow down and catch your breath before diving... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...

  17. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org...

  18. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...gave me a place to go once a week to get recharged. The fun change of pace helped break up the negativity...BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and...

  19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...260 nm, and 280 nm. Nucleic acids exhibit a characteristic absorption spectra across this range of wavelengths...absorption at 260 nm (A260). Figure 1: Characteristic absorption spectra for pure DNA and likely ...phenol, or guanidine salts, exhibit their own characteristic absorbance spectra, which you can detect by...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... an unusual type of IgG antibody called a heavy chain antibody (HCab), which are unique to camels, llamas...and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit nanobodies. ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...
Showing: 441 - 460 of 807 results