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We narrowed to 800 results for: cha;

Showing: 441 - 460 of 800 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...publications. Bonus: papers have to be summarized in 280 characters! Using Twitter to find papers  You’ll need to...a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional...

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...by the general public (either through taxes or charitable donations) so there is an increasing realization...funding or not; applications for funding from charities, commercial organizations, or universities may...-doc at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...will be greatly reduced, allowing for clearer characterization of the location, morphology, and circuit mapping...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... protein aggregation, you may want to consider changing your incubation temperature. Some proteins aggregate...kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane size is probably the easiest troubleshooting...alcohol. The increased SDS will increase the negative charge on the protein, allowing it to move more readily...Posttranslational modifications Check literature Highly charged amino acids Run control protein (if available...

  5. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... be translated at the ribosome into polypeptide chains that make up the primary structure of proteins.... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing PAM... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...individuals in your team you will have a much better chance of creating a high-performing team - which is every.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... about his/her background. This will give you a chance to determine how to tailor your presentation so...sought out your poster, but will also give you a chance to slow down and catch your breath before diving... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...

  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org...

  11. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...gave me a place to go once a week to get recharged. The fun change of pace helped break up the negativity...BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... an unusual type of IgG antibody called a heavy chain antibody (HCab), which are unique to camels, llamas...and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit nanobodies. ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons One of the CRISPR-transposon...in each type of CRISPR-Transposon system. (B) Mechanism of RNA-guided transposition in type I-F INTEGRATE...bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons. Insertion...

  15. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...adenosine to inosine in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to ...introducing a C opposite the target A to be edited. This change specifies the edit to be made when multiple As ... their RESCUE (RNA Editing for Specific C-to-U Exchange) system (Abudayyeh et al., 2019). To avoid some...

  16. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV expression...

  17. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using...Flexible. Low barrier to entry. Ample distribution channels both digitally and physically. Easy to incorporate...Media Medium to high Combines pros of multiple channels/balance out cons. Flexible. Can allow people to...

  18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...investigate viral processes in extreme detail. The mechanism of a particular observation can be easily studied...viral sequences (e.g. promoters) can be studied by changing the minigenome cis-acting sequences and monitoring...24093048. PubMed Central PMCID: PMC3787895. 6. Weik, Michael, et al. "The Ebola virus genomic replication promoter...

  19. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...host strain can help. In order to reduce the chance that the configuration of your fusion proteins ...J.L., Boles, E., André, D., and Frommer, W.B. K+ channel interactions detected by a genetic system optimized...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... and forth with. That being said, we probably exchanged dozens of drafts of the manuscript. This is the...transform the document into something cohesive-- change the sentences, make it flow, and start telling ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...
Showing: 441 - 460 of 800 results