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Showing: 441 - 460 of 756 results

  1. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also...verify that our sequencing results match sequence information contributed by the depositor, published backbone...region is present in the plasmid, we might need to perform a restriction digest to confirm the size of the...

  2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your...scientists are finding more and more ways to creatively perform selections in plasmid cloning. Additional resources...

  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...a Biorender watermark, which is usually ok for informal settings like lab meeting presentations. You can...collections of images are easy to download in a .png file format. Images are under a Creative Commons 3.0 license...good idea to double-check the image’s license information before using it. Have a tip for using one of ...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...Cong Read Addgene's CRISPR guide for background information on CRISPR/Cas9 systems Peruse the most recent...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...will spend 10 or more years with a small number of formal advisors learning how to be a scientist. It is ...willing to talk by phone. Don’t use email to get information if you can help it. Observe as much as you can...If lab members, neighbors or alumni give you information about the PI that concerns you, don’t just brush...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out Addgene...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...

  7. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...1970s by Alex van der Eb in the Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc...Resources References Shaw G., et al., Preferential transformation of human neuronal cells by human adenoviruses...0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors...

  8. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...these pieces aren't always appropriate for your formal scientific communications, they are fantastic fodder...Second, the introduction needs to provide enough information so that the audience can understand the conflict...scientific problem) will also help you determine what information is necessary and what can be left out, which ...

  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...collaboration without having to worry about proprietary information Work hours are long, but there is more flexibility...opportunities—projects end and layoffs occur, but good performers move around easily  Table 1: Taking it a Step... career with advancement in one company, good performers in for the long haul Limited movement in one...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... DOI Citation Formatter Just enter the DOI of a paper and select your desired formatting style. This tool...to get those words out and onto the screen and transform your work into a paper or a grant. Here’s a list...

  11. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...Perhaps you have a paper, protocol, or other information that may be helpful or of interest to them. Don...you to someone who you need to speak to for informational or career purposes.  Don’t only talk about work... conference soon? Get tips here Learn about informational interviews ...

  13. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...demonstrates that you have advanced writing and formatting skills, which are useful to many employers. Increasingly...Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer Journal Editor...are willing to exercise these skills outside of a formal work environment also shows that you are passionate...

  14. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... like gel electrophoresis to separate DNA or transforming cells with green fluorescent protein, these ... into a host cell (through a process called transformation), essentially giving the cell all the instructions...hands-on educational activities using the BioBits platform, please get in touch with us! We’ll work with ...

  15. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...participants, clarification - new and useful information, and invigoration - a renewed energy for continuing.... This keeps our all-company meetings short, informative, and pleasant. Email: Sometimes, the right ...Before long, you will be running collaborative, informative, energizing meetings all your own. Additional...

  16. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...You can’t delegate the giving of feedback or performance review. You can’t delegate tasks assigned to ... take on all tasks that involve confidential information. No matter who you delegate to, or what tasks...ensure successful results.  Can you provide the information and resources needed for the assignment? Setting...

  17. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...uncut DNA (lane 2) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks...make it easier for you to obtain a useful and informative diagnostic restriction digest. For your digest...fact that the circular DNA takes on several conformations the most abundant being: supercoiled, relaxed...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...improving access to useful research materials and information. References and Resources Additional Resources...

  19. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...therefore will require crosslinking, which uses formaldehyde or UV light to create strong but reversible ...actively interacting with, reducing background information. Your options for this step are sonication, which... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

  20. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...-based method for screens. Cas12a’s ability to perform multiplex editing within a single expression cassette...used to create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library...
Showing: 441 - 460 of 756 results