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We narrowed to 833 results for: ins

Showing: 441 - 460 of 833 results

  1. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant form...Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl...Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM...Yamanaka's work has been profound, and this is a rare instance in which "profound" seems like an understatement...degeneration; Laurenz Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking...clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ... Eric J. Perkins...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...searching the interwebs for lab resources, and instead conveniently provide them on your smart phone. ...holds the Gibco Cell culture manual and basic instructional videos. The search function in the manual just...that require restriction enzymes or PCR. The app contains a double digest tool that is only helpful for ...for NEB restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a...draw an amino acid or peptide for some reason. It contains all twenty amino acids plus selenocysteine and...

  4. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...certified health insurance executive (CHIE). He is a member of both the Internal Institute of Auditors and... Directors brings complementary experience and insight to the now 10 member board at Addgene. Let’s meet...at both MetLife & Ernst & Young, LLP, auditing insurance, finance and high-tech industries. He has earned...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

  5. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... thanks to our guest blogger Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager...post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford...your network to your advantage  As the profession gains more traction in the sciences, I believe that more...customer in mind. You can follow her on twitter @rgrubinstein. Additional resources on the Addgene blog Discover...

  6. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...AAV? A: One potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however...possible to make an efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid?...bringing total genome length to ~4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5...experiments using PHP.eB in the brain give some insight into codelivery efficiency at different doses. ...tropism to other serotypes, but reproducible success remains elusive.  There has also been a lot of effort in...

  7. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a short and accessible...information? This can be physical or digital. For instance, you would present information differently in ...image on a protocol page on our website, as an instructive step-by-step guide.  Finally, when writing your...after centrifugation and the syringe needle is inserted bevel-up at the interface between the 40% and ...captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the 40% layer containing...

  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The most obvious...could be a microscope, plate reader, or any other instrument which measured GFP levels and converted that ...includes tension monitors associated with specific proteins of interest such as actinin. When tension is high....2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service: Biosensor AAV Viral...Epigenetics with a FRET Based Biosensor Fluorescent Proteins 101: Fluorescent Biosensors ...

  9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...awards to early career scientists using fluorescent proteins in their research. These awards help early career...biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore that are susceptible... to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure 2: ...changes following opioid withdrawal, providing insight into new methods for treating patients in recovery...indicator which had improved performance in live mouse brains. Currently, she is using the improved voltage indicator...

  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...discussions.  Pro tip: Instead of connecting with someone you don’t really know, follow instead of connecting....information available. When I am speaking at a new institution, for example, I will often see a whole bunch ... “a postdoc from this Uni viewed your profile” instead of “Joanne Kamens, PhD viewed your profile.”  The...option for sending LinkedIn messages to Click Send instead of Press Enter to Send. That way you can type a...

  11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored a three-part...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...in the group was able to make it physically to instruct the workshop at the conference, but because each...of the scientific community. Lessons learned Instead of continuously talking about a problem and potential...

  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...show frustration if they're incorrect; take it instead as valuable feedback.  Listen. Think about the ...strategy. Don’t assume it’s the audience’s fault; instead look for ways to improve how you're communicating...If something I was communicating didn’t click, instead of blaming the audience, I would think about how... wasn't. Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...for every assay. Antibodies that are generated against a linear antigen tend to work very well for applications...application. Take immunohistochemistry (IHC), for instance. While an antibody may be validated for IHC, not...region highly conserved across species? If the proteins are highly homologous between your target species... each isotype or species to easily discern the proteins in your assay.  Narrowing the field After working...compare the staining patterns of multiple antibodies against the same target; tagged protein strategies that...

  14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive...the degradation rate of radio-labeled long-lived proteins as a proxy for autophagy. Cells are cultured with...washout period removes radio-labeled short-lived proteins primarily via proteasome degradation. Autophagy... to giving more fine-grained detail about what proteins or organelles are being degraded) and it is slow...

  15. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...destination vector + insert may spontaneously religate, this transient construct retains functional Type IIS...in the final construct. The destination vector contains sites with complementary overhangs that direct...complete with helpful tips and tricks, see The Sainsbury Lab website or Engler & Marillonet. Golden Gate...technology has adapted Golden Gate cloning for inserting the appropriate oligonucleotides specifying a ...

  16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...mutation in the evolved TadA domain. Because ABE7.10 contains a heterodimeric fusion of wild-type TadA and TadA7.10... the evolved TadA region. The ABE8.x-m version contains only the evolved TadA and is ~500 base-pair smaller...evaluate further.  The creation of accidental insertions and deletions (indel) by the catalytically impaired...more than 90% compared to ABE7.10. Choosing Cas9 proteins to increase the targeting scope of adenine base... Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: NG) to create NG-ABE8...

  17. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...challenge is finding relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight...search that’s constantly updated. This Twitter list contains >300 Twitter literature bots, including a bot ...AI-backed literature search developed by the Allen Institute for Artificial Intelligence. Semantic Scholar ...Initiative. Meta is not a traditional search engine. Instead, you create your own research feed by entering ... papers are published, similar to Facebook or Instagram. Key features Setting up a personalized feed is...

  18. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...first make your poster. While a poster generally contains the same sections as a primary research article...in your poster. This is a section where you can insert your references and contact information, and it...conclusions, and acknowledgements sections are inserted. The advantage of this style is that it will display... a poster vertically rather than horizontally. Instead of displaying your information as columns, you ... poster, written and graphical information are inserted into larger circles or ovals, that are arranged...

  19. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... they bind to a class of Argonaute proteins. The Argonaute proteins remove one strand of the siRNA and...cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid backbone. The...the process is a little more complicated. These strains are more picky eaters and refuse to eat liver paste...Sánchez lab protocol also provides step-by-step instructions for the “the bravest and more curious” students...

  20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...distinct proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic domains tethered...are a number of drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that..., Charlton et al. 2020). Most importantly, TET proteins affect transcription independent of their enzymatic...overlap the CpG of interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1...
Showing: 441 - 460 of 833 results