We narrowed to 794 results for: mpl

...only occur through editing by a APOBEC-Cas9n-UGI complex. So how exactly does this work? Figure 1:...DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create...DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction of ... single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038...

...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors... daughter cell during cell division. They don’t simply rely on chance (or random diffusion) to make this...some types low copy plasmids during cell division simply does not allow for enough time for correct partitioning...

...camera or tracking software. Locomotion is a highly complex process on the level of individual neurons and ...motivational and other aspects may be involved. For example, an animal moving around to explore the environment...the elevated plus maze, the light-dark box is a simple device comprised of a brightly lit (risky) and ...behavioral neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior...

...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA... the 20 amino acids and three stop codons. (B) Example of codon usage among different organisms. There...prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth codon optimizing a human gene ...optimized for expression in mammalian cells. For example, Henry Lester’s lab optimized the C. elegans GluCl...

...become resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species...Figure 1): Over-prescription of antibiotics (for example, giving antibiotics for an infection that isn’t...treatment regiment might wipe out bacteria that are completely susceptible to a drug while a full course of ...likely to develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s...

...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...when it is important to check their identity. For example, perhaps a working cell bank has been made by a...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...passage number, cell line, and date, which you complete each time you passage the cells (as well as recording...

...often have absolute position requirements. For example, a KDEL ER retrieval sequence only functions at... even disrupt fusion protein localization. For example, the formation of inappropriate inter-chain disulfide...disulfide bonds between FPs blocks Golgi complex-localized fusions from properly exiting the ER and fluorescing... that can be used in various combinations. For example, with standard fluorescent microscope filter sets...

...proteins. lon protease: Strains where this is completely deleted (designated lon or Δlon) similary reduce...variety of sources, so fear not—there are a few simple troubleshooting measures that can help get you ...will not express in an IPTG induction strain for example, nor will a p15 plasmid be compatible with a pLys...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and...development. Integrin structure and conformational complexity There are 24 known mammalian integrins, all heterodimers...demonstrated that anti-integrin αVβ5.9 “essentially completely blocks all adhesion,” inhibiting the function...Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams employed IPI synthetic antigen-binding fragments (Fabs)...

...scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...applications of scFvs: The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein.....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute... about the SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein....

...protein is cleaved by proteases. Nowadays, with ample structural information available, it’s clear that... better preserves the function of the POI. For example, the APT1-mVenus fusion localizes at the Golgi,... interact with other proteins or biomolecules. Examples of the successful insertion of a fluorescent protein...interactions) can be challenging. If a fusion can complement a knock-down or knock-out of the corresponding...

...important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS ...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

...build suspense. Notice the simplicity of their slides and how the slides complement the spoken message. Learn...get people to remember you). What better way to accomplish all of these things naturally than to give a ...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...what works great in one system or cell type can completely fail in a different system (Issa et al., 2023...postsynaptic transmission (Oh et al., 2020). For example, AAV1 and AAV9 tend to spread farther from the ...Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently express TH - so you...

...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that...

...genetic code of living organisms and its societal implications even before the discovery of genes themselves...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

...Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins that, in...photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected to a laser...Abdallah et al., 2018; Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors... Han, 2016). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could...

...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...another (e.g. green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek...trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

...April because devaluing science has alarming implications for our public policies—many of which impact..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...funding, and teaching experience that it takes to complete your resume, without ever thinking that research...research scientist would be a career that could simply cease to exist. But then November hit and panic ensued...

...,000 plasmids, kits, and viral service items we employ dozens of pieces of energy-intensive lab equipment...certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide... new programs, we're always striving to set an example of environmental stewardship.  Additional resources...